Zinc Finger Nucleases
Zinc finger nucleases (ZFNs) are artificial restriction enzymes, created by fusing an engineered zinc finger DNA binding domain
(which targets a desired sequences) with the DNA cleavage domain of FokI, a type II restriction enzyme.
| Supplier |
|---|
| ToolGen-LabOmics |
| Product |
| ZFN pairs service |
Features
- Genome Engineering: Gene knock-out, insertion, mutation, correction or deletion
- Targeted Genome edition: Designed to target precise, specific locus
- Complete knockout of any target gene
- No antibiotic selection required
- Biallelic modifications occur frequently
- Ready to use: No labor-intensive E. coli-based pre-screening steps
- Validated activity
- Affordable price
ZFNs are engineered DNA-cleaving enzymes made by fusing tailor-made DNA-binding zinc finger arrays to the FokI nuclease domain. ZFNs induce site-specific double strand breaks (DSBs) in DNA at user-defined locations, which leads to targeted modification of the genome. Recent studies have established ZFNs as an innovative tool for targeted genome editing in diverse cell lines and organisms (method of the year 2010 - Nature Methods Dec 2010). Because the zinc finger domain can be designed to recognize and introduce DNA double-strand breaks specifically in desired sequences in the genome, ZFNs provide an innovative tool for in vivo genome editing. Using our custom-synthesized ZFNs, scientists can knock out any gene of interest or edit its DNA sequence in cultured cells or other model systems such as zebrafish, mouse, rat, plants, etc.
Fig. 1. Principle of ZFN targeting and cleavage.
ZFNs make various types of genome editing possible, including, but not limited to, (1) the introduction of mutations into a gene of interest to knockout its function, (2) the deletion or insertion of genetic information at specific loci, and (3) the induction of large scale genetic deletions, duplications, and inversions. These types of genome editing were difficult, if not impossible, to achieve before ZFNs were developed; the ability to undertake such editing represents a unique opportunity in both basic and pharmaceutical research to translate our growing understanding of the genetic basis of many biological and pathological processes into industrial and therapeutic breakthroughs.
The ZFN service includes the design of ZFNs that target your gene of interest using our proprietary computer algorithm and synthesis of a carefully chosen set of ZFNs by the modular assembly method. Our ZFN custom service will allow you to start your experiments with a set of well-designed, ready-to-use ZFNs, bypassing the need for labor-intensive ZFN design and synthesis efforts in your own lab.
Fig. 3. Various types of genome editing through the use of ZFNs
Fig. 1. Principle of ZFN targeting and cleavage.
ZFNs make various types of genome editing possible, including, but not limited to, (1) the introduction of mutations into a gene of interest to knockout its function, (2) the deletion or insertion of genetic information at specific loci, and (3) the induction of large scale genetic deletions, duplications, and inversions. These types of genome editing were difficult, if not impossible, to achieve before ZFNs were developed; the ability to undertake such editing represents a unique opportunity in both basic and pharmaceutical research to translate our growing understanding of the genetic basis of many biological and pathological processes into industrial and therapeutic breakthroughs.
The ZFN service includes the design of ZFNs that target your gene of interest using our proprietary computer algorithm and synthesis of a carefully chosen set of ZFNs by the modular assembly method. Our ZFN custom service will allow you to start your experiments with a set of well-designed, ready-to-use ZFNs, bypassing the need for labor-intensive ZFN design and synthesis efforts in your own lab.
Fig. 3. Various types of genome editing through the use of ZFNs
The ZFN service includes the design of ZFNs that target your gene of interest using our proprietary computer algorithm and synthesis of a carefully chosen set of ZFNs by the modular assembly method. Our ZFN custom service will allow you to start your experiments with a set of well-designed, validated, ready-to-use ZFNs, bypassing the need for labor-intensive ZFN design and synthesis efforts in your own lab.
Genome Editing
-Gene Knock-out
-Gene Knock-out / Tagging
-Gene Insertion
-Gene Deletion
-Gene Mutation
-Gene Correction
-Gene Knock-out
-Gene Knock-out / Tagging
-Gene Insertion
-Gene Deletion
-Gene Mutation
-Gene Correction
1. Identify the gene target of interest:
Gather as much as possible of the following information: Gene Description, species, Nucleotide Accession, Entrez Gene ID, Symbols/Aliases or Catalog/product ID. You can also submit a sequence so we can search by target gene sequences homology (BLAST)2. Fill out the order form:
and fill with requested information. Send it back to us by email or FAX. We will contact you shortly to confirm: sequences, vectors and related products
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