Cas9 stable cell lines

CRISPR-Cas9 stable cell lines


Stable integration at the Human AAVS1 Safe Harbor site ensures consistent, stable expression of the CRISPR-Cas9 nuclease. Cas9 expression from this locus exhibits high activity, as shown by the T7 Endonuclease I mismatch cleavage assay (Figure 2).

Figure 2. Demonstration of stable Cas9 activity in a cell line. Human H1299 cells stably expressing Cas9 nuclease from the AAVS1 Safe Harbor site were transfected with a plasmid carrying an sgRNA for the HUWE I gene. 24 hours post transfection, cells were harvested and used for genomic DNA extraction. Using the GeneCopoeia IndelCheck™ kit, PCR products flanking the sgRNA target site were generated, denatured, reannealed, and treated with (+) or without (-) T7 Endonuclease I, which cleaves mismatched DNA. The presence of two bands below the uncut band at the correct sizes indicates that Cas9 is active in these cells.

 

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  1. Human cell line H1299 stably expressing CRISPR Cas9, single clone
    1 550,00 €
    Human cell line H1299 stably expressing CRISPR Cas9, single clone (indicated price is for Academic; Industrial customers please inquire) Learn More
  2. Human cell line MDA-MB-468 stably expressing Cas9 from random site
    1 550,00 €
    Human cell line MDA-MB-468 stably expressing CRISPR Cas9 (indicated price is for Academic; Industrial customers please inquire) Learn More
  3. Human cell line T47D stably expressing Cas9 from random site
    1 550,00 €
    Human cell line T47D stably expressing CRISPR Cas9 (indicated price is for Academic; Industrial customers please inquire) Learn More
  4. Human cell line SK-BR-3 stably expressing Cas9 from random site
    1 550,00 €
    Human cell line SK-BR-3 stably expressing CRISPR Cas9 (indicated price is for Academic; Industrial customers please inquire) Learn More
  5. Human cell line HCC70 stably expressing Cas9 from random site
    1 550,00 €
    Human cell line HCC70 stably expressing CRISPR Cas9 (indicated price is for Academic; Industrial customers please inquire) Learn More
  6. Human cell line DU4475 stably expressing Cas9 from random site
    1 550,00 €
    Human cell line DU4475 stably expressing CRISPR Cas9 (indicated price is for Academic; Industrial customers please inquire) Learn More
  7. Human cell line HCC1428 stably expressing Cas9 from random site
    1 550,00 €
    Human cell line HCC1428 stably expressing CRISPR Cas9 (indicated price is for Academic; Industrial customers please inquire) Learn More
  8. Human cell line CAMA-1 stably expressing Cas9 from random site
    1 550,00 €
    Human cell line CAMA-1 stably expressing CRISPR Cas9 (indicated price is for Academic; Industrial customers please inquire) Learn More
  9. Human cell line HCC38 stably expressing Cas9 from random site
    1 550,00 €
    Human cell line HCC38 stably expressing CRISPR Cas9 (indicated price is for Academic; Industrial customers please inquire) Learn More
  10. Human cell line AU-565 stably expressing Cas9 from random site
    1 550,00 €
    Human cell line AU-565 stably expressing CRISPR Cas9 (indicated price is for Academic; Industrial customers please inquire) Learn More

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