Donor plasmid design & synthesis
Donor plasmid design and synthesis for Knock-In (KI) and Gene Correction through Homologous Recombination (HR). To be used with engineered nucleases (ZFNs, TALENs and RGENs/CRISPRs)
| Supplier |
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| LabOmics |
| Product |
| Homologous Recombination Donor Plasmid Design and Synthesis |
Features
- Use with ZFNs, TALENs or RGEN /CRISPR for Knock-In: A DNA plasmid donor is required to perform Gene Correction and Knock-In experiment taking advantage of the HR repair mechanism after ZFN, TALEN or RGEN/ CRISPR induced DSB
- DNA plasmid donor design: careful design which takes into account DSB site, homologous arms optimal lengths, preventing undesired NHEJ repair mutations, avoiding undesired frame shifhts, etc.
- Introduces mutation, correction, tag or a whole expression cassette at locus of interest.
- Full synthesis sequence verified recombination sequence synthesis, delivered in small plasmid backbone
Homologous Recombination (HR) is an error-free DSB repair mechanism that copies genetic information from a homologous segment of DNA (e.g., from the sister chromatid). When Engineered Nucleases (eg. ZFNS, TALENs or RGEN/CRISPR) are transferred into a cell with a donor DNA with appropriate homologous sequences, HR will use the donor DNA to repair DSBs induced by engineered nucleases, which will result in the faithful transfer of genetic information from the donor DNA to the target genomic locus. Researchers can utilize this pathway to specifically insert sequence variations (e.g., single nucleotide polymorphisms, mutations) or genes of interest (e.g., genes encoding fluorescence proteins, antibiotic-resistance genes) into the target locus.
Fig. 1. Genome Engineering: Digestion of a target locus by an engineered nuclease pair triggers cellular double-strand break (DSB) repair machinery to recover the integrity of the genome sequence. Non-homologous end joining (NHEJ) and homologous recombination (HR) are the major DSB repair pathways in eukaryotic cells.
Fig. 1. Genome Engineering: Digestion of a target locus by an engineered nuclease pair triggers cellular double-strand break (DSB) repair machinery to recover the integrity of the genome sequence. Non-homologous end joining (NHEJ) and homologous recombination (HR) are the major DSB repair pathways in eukaryotic cells.
DNA Donor Plasmid design and synthesis
- Taylor made design according to the Knock-In or gene correction/mutation project
- Careful design which takes into account DSB site, homologous arms optimal lengths, preventing undesired NHEJ repair mutations, avoiding undesired frame shifhts, etc.
- Recombination cassette full synthesis, sequence verified.
- Delivered in a small plamsid backbone to minimize DNA mass.
-Knock-In or gene correction/mutation
Introduces mutation, correction, tag or a whole expression cassette at locus of interest through HR DSBs induced repair mechanism. The DNA donor plasmid should be co-transfected/ Co-injected with ZFNs, TALENs or RGEN/CRISPR.
Introduces mutation, correction, tag or a whole expression cassette at locus of interest through HR DSBs induced repair mechanism. The DNA donor plasmid should be co-transfected/ Co-injected with ZFNs, TALENs or RGEN/CRISPR.
DNA Donor Plasmid design and synthesis
Please submit your project at info@labomics.com, our qualified molecular biologists team will contact you to start evaluating feasability and costs.
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