FFPE RNA (including microRNA) Purification Kit

In stock
SKU
1025300
323,00 €
FFPE RNA (including microRNA) Purification Kit
Supplier
Norgen Biotek
Cat. no.
1025300
Kit size
50
Links
Norgen Biotek
Protocol
Information Sheet

Features

  • High quality and integrity of the isolated RNA - The purified total RNA is of the highest quality and integrity, and can be used in any sensitive downstream applications.
  • Isolate a diversity of RNA species - All RNA species can be isolated, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA).
  • No phenol:chloroform extractions - Total RNA is isolated from FFPE tissue samples without the use of harmful chemicals such as phenol or chloroform.
  • Rapid procedure - Isolate total RNA from FFPE tissue sections using a rapid spin column format.

Norgen's FFPE RNA Purification Kit provides a rapid method for the isolation and purification of total RNA (including microRNA) from formalin-fixed paraffin-embedded (FFPE) tissue samples. Using formalin to fix tissues leads to crosslinking of the RNA and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the RNA over time. Norgen’s FFPE RNA Purification Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of RNA. The kit is able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as fragmentation of the RNA is known to occur over time .
Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. The RNA is preferentially purified from other cellular components without the use of phenol or chloroform. The process first involves deparaffinization of the FFPE samples through a series of xylene and ethanol washes. Next, the FFPE samples are digested with the provided Proteinase K and Digestion Buffer. Binding Solution and ethanol are then added to the lysate, and the solution is loaded onto a spin-column. Norgen’s resin binds nucleic acids in a manner that depends on ionic concentrations, thus only the RNA will bind to the column while other contaminants will be removed in the flowthrough or retained on the top of the resin. At this point, any remaining traces of genomic DNA can be digested using an optional protocol, allowing for pure RNA samples to be isolated. The bound RNA is then washed with the provided RNA Wash Solution in order to remove any impurities, and the purified total RNA is eluted with the Elution Buffer.
Column Binding Capacity 50 µg
Maximum Column Loading Volume 600 µl
Size of RNA purified All sizes
High Quality and Yield of Total RNA
Total RNA was isolated from equal amounts of an FFPE tissue sample using Norgen’s FFPE RNA Purification Kit and a leading competitors kit. The purified RNA was then resolved on an Agilent BioAnalzyer. As it can be seen, Norgen not only isolated higher yields of total RNA, but the RNA was also of a higher quality as evidenced by the higher RIN values obtained with Norgen’s RNA.

High Yield of a Diversity of RNA Species
Total RNA was isolated from equal amounts of an FFPE tissue sample using Norgen’s FFPE RNA Purification Kit and a leading competitors kit. The purified RNA was then used as the template in a RT-qPCR for detecting the beta-actin gene (Panel A) and for detecting miR-21(Panel B). In both graphs the blue lines correspond to Norgen isolated-RNA and the green lines correspond to competitor-isolated RNA. As it can be seen, Norgen’s kit isolated higher yields of RNA in both cases, as indicated by the higher C T values of the blue lines. Also, Norgen’s kit successfully isolated not only large RNA (Panel A) but also microRNA (Panel B), indicating the diversity of RNA species isolated.


  • qPCR
  • qRT-PCR
  • primer extension
  • expression array assays
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