Total Protein Concentration and Detergent Clean-Up Maxi Kit

In stock
SKU
1017100
138,00 €
Total Protein Concentration and Detergent Clean-Up Maxi Kit
Supplier
Norgen Biotek
Cat. no.
1017100
Kit size
4
Links
Norgen Biotek
Protocol
Information Sheet

Features

  • No detergent carryover - Unlike HPLC procedures, each sample is processed individually using its own spin tube providing no carryover or column bleed from sample to sample.
  • Useable with a variety of detergents - The kit can be used to remove detergents including SDS, Triton® X-100, CHAPS, NP-40, and Tween 20.
  • Effective detergent removal - Removes more than 95% of detergents for many protein samples, allowing tryptic digestion.
  • High recovery - Protein recoveries of up to 100% (using initial detergent concentrations of 0.1% and 1.0%) for many proteins.
  • Fast and easy processing - In contrast to longer dialysis procedures, this easy-to-use procedure allows processing of a number of samples in about 40 minutes.

The ProteoSpin™ Detergent Clean-Up Kit provides a fast and simple procedure for the removal of SDS, Triton® X-100 and other detergents from protein samples. Detergents are extensively used to prepare protein samples; however, these detergents must often be removed prior to downstream analysis because of their undesirable effects. These include extraneous peaks in mass spectrometry, artifacts with chromatography and electrophoresis, interference with microinjection into cells and interference with protein immunization. The ProteoSpin™ Detergent Clean-Up Maxi Kit can remove greater than 95% of detergents from protein samples while maintaining high protein recovery. The kit is able to remove all types of detergents including ionic, non-ionic and zwitterionic detergents. It is designed to remove detergents in protein solutions either in their free form or bound form, as when complexed with the protein.
Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. The purified protein can be used in a number of downstream applications including mass spectrometry, SDS-PAGE, proteolytic digestion, isoelectric focusing, x-ray crystallography, and NMR spectroscopy. The ProteoSpin™ Detergent Clean-Up Maxi Kit contains solutions for the processing of both acidic and basic protein samples. Each spin column is able to process detergent-containing samples from 0.25 – 8 mg of protein. Detergents including SDS, Triton® X-100, CHAPS, NP-40 and Tween 20 can be removed using the kit, with protein recoveries of 80 – 95% for most proteins. The kit has a shelf life of at least 1 year when stored as suggested. Each ProteoSpin™ Detergent Clean-up Maxi Kit provides essential components for use with the provided protocols. Column Activation and Wash Buffer for Acidic Proteins pH Binding Buffer for Acidic Proteins Column Activation and Wash Buffer for Basic Proteins pH Binding Buffer for Basic Proteins Elution Buffer Neutralizer Maxi Spin Columns Elution Tubes Short Protocol Card Application Manual
Maximum Protein Input 8 mg
Protein Recovery Up to 100%
Minimum Protein Input 0.25 mg
% Detergent Removal > 95%
Minimum Elution Volume 4 mL
Time to Process 4 Samples 40 minutes
Efficient Removal of SDS

High Recovery of Proteins from an SDS-Containing Solution

  • Mass spectrometry
  • SDS-PAGE
  • Proteolytic digestion
  • Isoelectric focusing
  • X-ray crystallography
  • NMR spectroscopy
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