ProteoSpin Urine Protein Concentration Micro Kit

In stock
SKU
1017400
245,00 €
ProteoSpin Urine Protein Concentration Micro Kit
Supplier
Norgen Biotek
Cat. no.
1017400
Kit size
25
Links
Norgen Biotek
Protocol
Information Sheet
MSDS

Features

  • Fast and easy processing - Efficiently process up to 12 samples in only 20 minutes using an easy-to-use protocol.
  • Complete Kit - All columns and solutions required are provided.
  • No molecular weight cutoff - Based on an ion exchange mechanism, a broad size range of proteins can be processed.
  • Proteins bind while salts are discarded in flowthrough - Norgen's resin has an inherent low affinity for salt, but high affinity for proteins, providing salt removal and buffer exchange simultaneously.
  • Suitable for downstream applications - Final elution suitable for: SDS-PAGE, 2D gel electrophoresis, Whole protein mass spectrometry, Protein microarrays.

Urine protein analysis can be used for the identification of potential biomarkers in urine, and to diagnose and/or monitor renal and other diseases. Due to the fact that urine can be collected non-invasively in large amounts, it provides an attractive alternative to blood plasma as a potential source of disease biomarkers. The ProteoSpin ™ Urine Protein Concentration Micro Kit provides a fast and simple procedure for concentrating dilute solutions of urine proteins from small volumes of urine.
Purification is based on spin column chromatography using Norgen’s proprietary resin as an ion exchanger. Urine proteins will bind to the column, while non-specifically bound materials such as salts are easily removed from the sample. The simultaneous removal of salts while concentrating a dilute urine protein solution makes the kit a convenient method for preparing proteins before running downstream proteomic applications including SDS-PAGE, 2D gels, whole protein mass spectrometry, and protein microarrays. The ProteoSpin™ Urine Protein Concentration Micro Kit contains sufficient materials for 25 preparations. Each spin column is able to concentrate and desalt up to 200 µg of urine proteins. Preparation time for 12 samples is only 20 minutes. The kit has a shelf life of at least 1 year when stored as suggested. Each ProteoSpin™ Urine Protein Concentration Micro Kit provides essential components for use with the provided protocols. pH Binding Buffer Column Activation and Wash Buffer Protease Inhibitor Mix Stabilizer Elution Buffer Neutralizer Micro Spin Columns Elution Tubes Short Protocol Card Application Manual
Maximum Urine Volume Input 1 mL
Maximum Recovered Protein 200 µg
Minimum Elution Volume 30 µL
Time to Process Multiple Samples 20 minutes for 12 samples
No Loss of Urine Proteins
No urine proteins are lost during the binding step, as can be seen by examining the binding flowthrough. Lane A is 25 µL of input urine, Lane B is the binding flowthrough, and Lane C is 15 µL of the elution.


Concentration of Urine Proteins
A dose response indicating the concentration of urine proteins from increasing input amounts of urine. Lane A represents 25 µL of an input urine sample, while lanes B to F are the elutions after the proteins were concentrated using the ProteoSpin™ Urine Protein Concentration Micro Kit. The input amounts of urine that were loaded onto each column were 100 µL, 200 µL, 300 µL, 400 µL, and 500 µL respectively.


  • SDS-PAGE
  • 2D gel electrophoresis
  • Whole protein mass spectrometry
  • Protein microarrays
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