Hot Firepol (Hot Start Taq DNA Polymerase) 500 U

In stock
SKU
6300500
69,00 €
HOT FIREPol® DNA Polymerase is a chemically modified FIREPol® DNA Polymerase. At ambient temperatures it is inactive, having no polymerization activity.
£63 / €69

Supplier
LabOmics Solis Biodyne
Cat. No
6300500
Pack size
500 units
Links
Data Sheet
MSDS

Features

  • inactive at ambient temperature, having no polymerization activity
  • is activated by a 15 min incubation step
  • prevents extension of nonspecifically annealed primers and primer-dimers formed at low temperatures
  • enhanced stability at room temperature: no activity loss for up to 1 month at RT
  • Shipped at RT with no activity loss: save on shipping costs

HOT FIREPol® DNA Polymerase is a chemically modified FIREPol® DNA Polymerase. At ambient temperatures it is inactive, having no polymerization activity. HOT FIREPol® DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during PCR setup. The enzyme has 5’→3’ polymerization-dependent exonuclease replacement activity but lacks 3’→ 5’ exonuclease activity.
  • Source: Purified from an E.coli strain that carries an overproducing plasmid containing a modified gene of Thermus aquaticus DNA Polymerase.
  • Concentration: 5U/µL
  • Unit definition: One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmol of dNTPs into an acid-insoluble form in 30 minutes at 74ºC.
  • Storage and Dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers.
  • Quality control: The enzyme is free of nicking and priming activities, exonucleases and non-specific endonucleases. SDS/PAGE - 95 kD band, >98% pure. Activity and stability tested via thermo cycling. The error rate per nucleotide per cycle is ~ 2.5 x 10-5; the accuracy is ~ 4 x 104. Estimated half life at 95ºC is 1.5 hours.
  • Shipping and Storage conditions: Routine storage: -20ºC Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol® DNA Polymerase.
  • Recommended PCR Buffers: Our standard buffers are HOT FIREPol® 10 x Buffer B2 and HOT FIREPol® 10 x Buffer B1 (detergent free). For further optimization please try our KCl based Buffer A1.
  • Mix Composition:
    • HOT FIREPol® DNA Polymerase
    • HOT FIREPol® 10 x Buffer A1 (Mg2+ and detergent free)
Tris-HCl and KCl
    • HOT FIREPol® 10 x Buffer B1(Mg2+ and detergent free)
Tris-HCl and (NH4)2SO4
    • HOT FIREPol® 10 x Buffer B2
Tris-HCl, (NH4)2SO4 and detergent
    • 25 mM MgCl2
    • 10 x Solution S
Additive that facilitates amplification of difficult templates (e.g. GC-rich DNA templates). This solution should be used at a defined working concentration (1x, 2x or 3x solution). Solution S is NOT a reaction buffer and should be used ONLY IF non-specific amplifications occur.
  • Hot Start PCR
  • DHPLC
  • TA cloning
Write Your Own Review
You're reviewing:Hot Firepol (Hot Start Taq DNA Polymerase) 500 U