Luc-Pair™ miR Luciferase Assay Kit (1000 rxns)

In stock
SKU
LPFR-M100
885,00 €
The Luc-Pair™ miR Luciferase Assay Kit provides an efficient system in a convenient 96-well plate format for measuring firefly and Renilla luciferases sequentially. Optimized for use with GeneCopoeia miRNA 3' UTR Target Sequence Expression Clones, Luc-Pai
Supplier
GeneCopoeia
Cat. no.
LPFR-M100
Kit size
1000 rxns
Links
miRNA Target clones

Features

  • High sensitivity in a dynamic range.
  • Successive reporter system.
  • Consistent reproducibility.
  • optimized luciferase reporter assay for use with miRNA Target Sequence 3' UTR Expression Clones.
  • Cost effectiveness.
  • Simple assay format.
  • Suitable for high-throughput screening.

The Luc-Pair™ miR Luciferase Assay Kit provides an efficient system in a convenient 96-well plate format for measuring firefly and Renilla luciferases sequentially. Optimized for use with GeneCopoeia miRNA 3' UTR Target Sequence Expression Clones, Luc-Pair™ miR assays, enable easy, cost effective validation of miRNA activity.
  • Ideal for GeneCopoeia miRNA 3' UTR Target Sequence Expression Clones
  • Easy to use -- less hands-on time and quick results
  • Suitable for micro-plate format -- save time and money
Measure inhibitory effect of a miRNA on a target sequence using the gene reporter system of miRNA 3' UTR Target Sequence Expression Clones:
Figure 1. The inhibitory effect of miRNA on a target sequence (3' UTR) expression clone can be measured with GeneCopoeia Luc-Pair™ miR Luciferase Assay Kit. HEK 293 cells were plated on a 6-well plate. On the second day, the cells were transfected with 1.0 µg of target sequence (3' UTR) expression clone (pEZX-MT01-Lin28 UTR-fLuc) and 1.4 µg of miRNA expression vector (or miRNA control vector) as indicated in the figure. The cells were transferred to a 96-well plate 18 hours after transfection and cultured for another 24 hours. Both firefly luciferase and Renilla luciferase activities were measured as described and data was recorded on Victor II machine. Firefly luciferase activity was then normalized with Renilla luciferase activities in the same well.

Linear range:


Figure 2. Linear relationship between emitted light and amount of luciferase expression vector transfected in HEK293. HEK 293 cells were plated on a 6-well plate. On the second day, the cells were transfected with different amounts of GeneCopoeia pMRO or pEZX-MT01 miRNA 3' UTR Target Sequence Clone as indicated in the figure. The cells were transferred to a 96-well plate 18 hours after transfection and cultured for another 24 hours. Firefly luciferase assay was then performed on pMRO vector (Fig. 2a) and Renilla luciferase assay was performed on pEZX-MT01 vector (Fig. 2b).

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