miTarget™ miRNA Target Sequence (3’ UTR) Expression Clones

Supplier
GeneCopoeia
Product
miTarget™ miRNA Target Sequence (3’ UTR) Expression Clones
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GeneCopoeia GeneCopoeia information

Features

• miTarget™ microRNA target sequence expression clones that cover 25,000 human and 20,000 mouse genes



• All 3’-UTR sequences in these expression constructs are curated from public domain gene sequence database



• 3’-UTR Sequences immediately after coding sequences are subsequently cloned after a reporter gene(firefly luciferase) in a mammalian expression vector



• The regulatory effect of a particular miRNA on its potential target sequences is assessed by the firefly luciferase functional assay.

MicroRNAs (or miRNAs) are small non-coding single-stranded RNA molecules that are transcribed from the genomes of eukaryotic organisms. They are highly conserved and usually 21~23 nucleotides in length. MiRNAs have been found to be involved in almost all cellular activities and functions. They regulate the expression of genes by binding to the 3’ untranslated regions (3’-UTRs) of targeted mRNAs. Irregularities in miRNA regulated gene expression have been found to be associated with cancers, cardiovascular disorders, and a variety of other diseases.
We are now offering miExpress™ Precursor miRNA expression clones for 750 human, 450 mouse, and 270 rat genes and miTarget™ microRNA target sequence expression clones that cover 25,000 human and 20,000 mouse genes
Researchers can use these miRNA expression clones to study the expression regulation of corresponding genes/proteins. In combination with target sequence (3’-UTR) expression clones, which have luciferase as the reporter gene, researchers can also cross-validate. We also offer multiple sets of full length human and mouse expression ready ORF cDNA clones that can be used for gain-of-function studies of corresponding target genes.

We offer genome-wide miRNA target sequence (3’-UTR) expression clones in a mammalian expression vector system. All 3’-UTR sequences in these expression constructs are curated from public domain gene sequence database. 3’-UTR Sequences immediately after coding sequences are subsequently cloned after a reporter gene (firefly luciferase) in a mammalian expression vector. The expression of the reporter gene firefly luciferase is controlled by SV40 promoter. A tracking reporter gene, renilla luciferase, is also constructed in the vector system, which provides tracking indicators for successful transfection and expression of these miRNA target sequence constructs in target cells. Figure 1 shows the basic features of such an expression construct. The regulatory effect of a particular miRNA on its potential target sequences is assessed by the firefly luciferase functional assay. These expression constructs transcribe a chimeric mRNA consisting of coding ORF sequence for firefly luciferase and a 3’-UTR target sequence. Upon binding of the 3’-UTR target sequence by a targeting miRNA, enzymatic activity of the luciferase encoded by the firefly luciferase ORF in the chimeric mRNA will be regulated accordingly. The effect can be quantified by the colorimetric luciferase assay.



Fig. 1. Vector features for human miRNA target sequence expression clones

• Gene expression regulation. The regulatory and functional effect of the miRNAs can be studied individually or systematically using the whole set or a subset of miRNA expression constructs by transduction of precursor miRNAs into target cells either via transfection or viral infection
• MiRNA target gene identification and validation. Together with GeneCopoeia’s miRNA target validation expression clones, miRNA expression constructs can be used to identify and verify their target genes
• References:

• Lagos-Quintana M, Rauhut R, Lendeckel W, Tuschl T; "Identification of novel genes coding for small expressed RNAs"; Science. 294:853-858(2001).
• Suh MR, Lee Y, Kim JY, Kim SK, Moon SH, Lee JY, Cha KY, Chung HM, Yoon HS, Moon SY, Kim VN, Kim KS; "Human embryonic stem cells express a unique set of microRNAs"; Dev Biol. 270:488-498(2004).
• Dostie J, Mourelatos Z, Yang M, Sharma A, Dreyfuss G; "Numerous microRNPs in neuronal cells containing novel microRNAs"; RNA. 9:180-186(2003).
• Michael MZ, O' Connor SM, van Holst Pellekaan NG, Young GP, James RJ; "Reduced accumulation of specific microRNAs in colorectal neoplasia"; Mol Cancer Res. 1:882-891(2003).
• Kasashima K, Nakamura Y, Kozu T; "Altered expression profiles of microRNAs during TPA-induced differentiation of HL-60 cells"; Biochem Biophys Res Commun. 322:403-410(2004).
• Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M, Weir DB, Choksi R, De Vita G, Frezzetti D, Trompeter HI, Hornung V, Teng G, Hartmann G, Palkovits M, Di Lauro R, Wernet P, Macino G, Rogler CE, Nagle JW, Ju J, Papavasiliou FN, Benzing T, Lichter P, Tam W, Brownstein MJ, Bosio A, Borkhardt A, Russo JJ, Sander C, Zavolan M, Tuschl T; "A mammalian microRNA expression atlas based on small RNA library sequencing"; Cell. 129:1401-1414(2007).
• Lui WO, Pourmand N, Patterson BK, Fire A; "Patterns of known and novel small RNAs in human cervical cancer"; Cancer Res. 67:6031-6043(2007).
• Marton S, Garcia MR, Robello C, Persson H, Trajtenberg F, Pritsch O, Rovira C, Naya H, Dighiero G, Cayota A; "Small RNAs analysis in CLL reveals a deregulation of miRNA expression and novel miRNA candidates of putative relevance in CLL pathogenesis"; Leukemia. 22:330-338(2008).

1. Identify the gene target of interest:

You can browse the entire miRNA Target Clones Collection:
human
mouse
rat

Or gather as much as possible of the following information: Gene Description, species, Nucleotide Accession, Entrez Gene ID, Symbols/Aliases or Catalog/product ID. You can also submit a sequence so we can search by target gene sequences homology (BLAST)

Alternatively, you can use our search tool above or the search tool by GeneCoppoeia - search miRNA Target(3'UTR) by Genecopoeia - and order the product ID result.

2. Fill out the order form:

and fill with requested information. Send it back to us by email or FAX. We will contact you shortly to confirm: sequences, vectors and related products