miRNA qPCR Arrays

Whole-genome or focused group profiling of miRNA expression using arrayed validated primers and robust RT-PCR conditions

The miProfile miRNA PCR Arrays are designed for profiling the expressions of pre-defined or customized sets of miRNAs in various tissues or cells. The resulting differential expressions of profiled miRNAs help researchers to identify those miRNAs that are of biological significance and importance relevant to their research. Each 96-well plate contains up to 84 pairs of PCR primers (forward: miRNA-specific primer; reverse: universal primer), which have been pre-validated and deposited in designated wells. Each plate also has 12 wells that contain different types of controls for monitoring the efficiency of the entire experimental process: from reverse transcription to qPCR reaction.

Features

• Genome-wide coverage, pre-arranged groups, or customized groups

  Largest genome-wide miRNA coverage, Cancer-related groups or Customized miRNA arrays for focused study

• Sensitive

  Detect miRNA from as little as 10 pg of small RNA or 20 pg of total RNA

• Specific

  Be able to distinguish miRNAs with single nucleotide mismatches. Each primer set has been experimental validated for specific amplification

• Broad linearity

  Allow miRNA at varieties of expression level to be detected simultaneously

• Reproducible

  High reproducibility (R2>0.99) for inter-array and intra-array replicates

• Validated miRNA primers

  Each miRNA primer is designed using a proprietary algorithm and experimentally validated