miArrest™ chemically synthesized miRNA inhibitors
chemically synthesized miRNA inhibitors
| Supplier |
|---|
| GeneCopoeia |
| Product |
| miArrest™ chemically synthesized miRNA inhibitors |
| Links |
| GeneCopoeia GeneCopoeia information |
Features
- 750 human, 450 mouse and 270 rat chemically synthesized miRNA inhibitors
- Chemically enhanced to improve efficiency and longevity
- Easier delivery by transfection or electroporation
Ideal for transient transfection, synthetic miRNA inhibitors are chemically-synthesized and sequence-specific single-stranded nucleic acid molecules that are designed and optimized to bind to specific miRNA molecules. Upon binding, they block the effect of miRNA molecules on their targeted genes with high specificity and efficiency.
Fig. 1. Simplified mechanism of action for vector-based miRNA inhibitors
Fig. 2. Data showing the effect of miArrest miRNA synthetic inhibitor in a dose-dependent manner Varying amounts of synthetic miR-125a inhibitor (GeneCopoeia HmiR-AN0094-SN were co- transfected into HEK 293 cells with 1) a miR-125a precursor expression plasmid (GeneCopoeia HmiR0309-MR03) and 2) a miRNA target sequence expression clone expression LIN28, a known target gene for miR-125a (GeneCopoeia HmiT019205-MT01: 3’-UTR sequence of LIN28 in FLuc-RLuc dual reporter expression vector). Both Firefly luciferase activity and an internal Renilla luciferease activity were determined 24 hours post-transfection. The activity ratio of FLuc to RLuc was set to 1 for the single transfection sample with LIN28 3’-UTR target sequence expression clone (left-most bar), against which the activities of other samples were normalized. The result shows that mir-125a suppressed the luciferase activity from the Fluc-LIN28-3’-UTR clone by more than 60% (Bar 2 from left). This suppression effect was blocked by the introduction of varying amount of miArrest™ inhibitor clone against miR-125a in a dose-dependent manner.
Fig. 1. Simplified mechanism of action for vector-based miRNA inhibitors
Fig. 2. Data showing the effect of miArrest miRNA synthetic inhibitor in a dose-dependent manner Varying amounts of synthetic miR-125a inhibitor (GeneCopoeia HmiR-AN0094-SN were co- transfected into HEK 293 cells with 1) a miR-125a precursor expression plasmid (GeneCopoeia HmiR0309-MR03) and 2) a miRNA target sequence expression clone expression LIN28, a known target gene for miR-125a (GeneCopoeia HmiT019205-MT01: 3’-UTR sequence of LIN28 in FLuc-RLuc dual reporter expression vector). Both Firefly luciferase activity and an internal Renilla luciferease activity were determined 24 hours post-transfection. The activity ratio of FLuc to RLuc was set to 1 for the single transfection sample with LIN28 3’-UTR target sequence expression clone (left-most bar), against which the activities of other samples were normalized. The result shows that mir-125a suppressed the luciferase activity from the Fluc-LIN28-3’-UTR clone by more than 60% (Bar 2 from left). This suppression effect was blocked by the introduction of varying amount of miArrest™ inhibitor clone against miR-125a in a dose-dependent manner.
- Study loss-of-function effect and uncover miRNA functions
- Identify and validate miRNA targets
- Elucidate the role of miRNA in cellular processes and pathological pathways, including cell differentiation and the formation of pluripotent stem cells
- Identification and validation of diagnostic and therapeutic biomarkers and targets
- See also:
1. Identify the miRNA of interest:
You can browse the entire Precursor miRNA Clones Collection:human
Or gather as much as possible of the following information: Precursor/Mature miRNA Name, Precursor/Mature miRNA Accession, Description or Catalog/product ID. You can also submit a sequence so we can search by Precursor or Mature Sequences homology (BLAST) and we will do the rest
Alternatively, you can use the search tool by GeneCoppoeia - Search miRNA clones/qPCR primers/Inhibitors by GeneCopoeia - and order the product ID result.
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