Amino-Adembeads 300nm 30ml

Amino-Adembeads 300nm 30ml
728,00 €

Availability: In stock

SKU : 2002232

Amino-Adembeads 300nm 30ml
Description

Details

£665 / €728

Supplier
Ademtech
Cat. No
2002232
Kit size
30 ml
Links
ademtech
Protocol Amino-adembeads 200nm.pdf
Protocol Amino-adembeads 300nm.pdf
Information Sheet

Features

  • Unique size distribution: CV
  • No sedimentation: due to the small size of the beads (200nm or 300nm)
  • High NH2 density: ≈ 100µmol/g
  • High surface area available for specific coupling up to 15 m2/g for the Amino-Adembeads of 200nm
  • Easy to couple: a complete procedure and buffers have been especially developped for protein coupling:
  1. via COOH protein group Amino 1 Activation Buffer code (Cat No 2010102) Storage Buffer (Cat No 2010201) EDC (carbodiimide, (Cat Nos 2010401, 2010402,2010403,2010404)
  2. via NH2 protein group Amino 2 Activation Buffer code (Cat No 10103) Storage Buffer (Cat No 2010201) EGDE (Ethylène Glycol Diglycidyl Ether, (Cat Nos 2010501, 2010502, 2010503, 2010504)

Amino-Adembeads are monodispersed and super-paramagnetic beads composed of magnetic core encapsulated by a hydrophilic polymer shell. The surface is activated with amine functionality. Amino-Adembeads are produced under asceptic conditions and are sold in an aqueous suspension containing 0.09% NaN3.
The functional amino groups of Amino- Adembeads offer the possibility for many different immobilisation procedures for use with proteins or other ligands via activation with carbodiimide or bi-functional reagents for example. We recommend coupling 10-50 µg of ligand per mg of beads. Protocols should be optimised to meet your requirements (e.g. sample volume, concentration of ligand / beads/ EDC).
Cat No 2002220 Functionality: NH2 Diameter: 200 nm Volume: 3 ml 1%
Cat No 2002221 Functionality: NH2 Diameter: 200 nm Volume: 15 ml 1%
Cat No 2002222 Functionality: NH2 Diameter: 200 nm Volume: 30 ml 1%
Cat No 2002223 Functionality: NH2 Diameter: 200 nm Volume: 60 ml 1%
Cat No 2002230 Functionality: NH2 Diameter: 300 nm Volume: 3 ml 1%
Cat No 2002231 Functionality: NH2 Diameter: 300 nm Volume: 15 ml 1%
Cat No 2002232 Functionality: NH2 Diameter: 300 nm Volume: 30 ml 1%
Cat No 2002233 Functionality: NH2 Diameter: 300 nm Volume: 60 ml 1%
Amino-Adembeads are designed to act as solid support for a wide variety of biomagnetic separations and manipulations. The surface amino groups allow for immobilization of ligands such as proteins, peptides oligonucleotides or other target specific molecules through activation with a large range of different bi-fuctional reagents and carbodiimide. Biomolecules containing aldehyde or ketone groups can also be immobilized via reductive amination without prior activation of the surface. Once coupled with ligand, the beads can be added to a cell lysate or other suspensions containing your target molecule. After a short incubation, the beads can be pulled to the side of the test tube by use of a magnetic device allowing aspiration of unbound material. Furthermore, the magnet facilitates washing and concentration of the isolated target.
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