Smart DNAdem-Kit for cells & tissues

In stock
SKU
2006121
228,00 €
Smart DNAdem-Kit for cells & tissues
£210 / €228

Supplier
Ademtech
Cat. No
2006121
Kit size
100 preps
Links
ademtech
Protocol
Information Sheet

Features

  • Capture and direct amplification: go directly to PCR and Real-time PCR without elution step
  • Simple and fast: less than 10 minutes (lysis step included) at room temperature
  • Higher recovery: no DNA lost during organic extraction and centrifugation and elution step
  • Greater sensitive compared with other technologies (spin column and organic extraction)
  • Work with small amounts of samples, 1mg of tissue
  • Reproducible system: less sample variability

The Smart D-N-Adem-Kit is a new system which is specially designed for the capture of gDNA and direct amplification on magnetic beads. Smart-Adembeads have a specific and proprietary polymer on their surface, designed for DNA capture by electrostatic interactions and compatible with direct PCR and Real-Time PCR without the need to perform an elution step. Animal tissues or cells are mixed with the Lysis Buffer. Smart-Adembeads bind specifically to the gDNA. Proteins and other contaminants are then eliminated in the washing step. The purified gDNA bound to the Smart-Adembeads can be used directly for PCR and Real-Time PCR analyses. The Smart D-N-Adem-Kit procedure allows cleaning gDNA in less than 10 minutes, lysis step included. DNA isolation is achieved without phenol, ethanol, chloroform and ionic chaotropes; thus the purified gDNA bound to the Smart-Adembeads demonstrates improved downstream performance in PCR and qPCR. Unlike other purification systems, no elution of the DNA is required, making it possible to maximize the amount of templates available for the reaction archieving greater sensitivity. This method makes this Kit ideal for processing small amount of samples where maximun DNA recovery is critical.
  • Sample Preparation:
1.From cells: Procedure described up to 5x105cells: Collect the cells and transfer them to a 1.5ml microcentrifuge tube in order to have them in a minimal volume of culture media or PBS (up to 1/5 of the Lysis Buffer volume). Add 50μl to 150μl of Lysis Buffer. Add 5μl of Proteinase K and 1μl of RNase. Mix by pipetting (or flick the tube) and incubate at room temperature for 5 minutes.
2.From animal tissues: Procedure described up to 25mg: Add 100μl of Lysis Buffer and 5μl of Proteinase K to a 1.5ml microcentrifuge tube. Flick the tube (or mix by pipetting). Immerse fresh or thawed tissues (up to 25mg) in the lysis solution and incubate at room temperature for 5-10 minutes. Remove and transfer the lysis solution to a new 1.5ml microcentrifuge tube. Add 1μl of RNase. Flick the tube (or mix by pipetting) and incubate at room temperature for 5 minutes.
  • DNA capture:
Add 1-5μl of homogenized Smart-Adembeads.
  • Add 50-150 μl of Binding Buffer and incubate at room temperature for 1 minute.
  • Place the tube on the magnet (also available from Labomics) for at least 1 minute and discard the supernatant.
  • Wash twice with 100μl of Washing Buffer
  • Place the tube on the magnet for at least 1 minute and discard the supernatant.
  • Resuspend beads in 10-50μl of Amplification Buffer. Use the final solution directly for PCR and Real-Time PCR
Beads 500µL Smart-Adembeads
Buffers 15 mL Lysis Buffer 15 mL Binding Buffer 20 mL Washing Buffer 5 mL Amplification Buffer
Storage Store at 2-8°C.


  • For DNA capture from cells and tissues and direct amplification without elution
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