RiboCellin is a powerful lipid based in vitro delivery reagent which allows to achieve higher delivery efficiencies of siRNA (or dsRNA) into cell lines and primary cells as compared to other lipid and polymer based delivery reagents. It allows performing very good and reproducible gene silencing with minimum amounts of siRNA and extremely low non specific effects.
|RiboCellin siRNA Transfection Reagent
Upon receipt, the kit should be stored at 4°C. Stored properly, the kit components should remain stable for at least 1 year.
Figure 1: RiboCellin is a powerful siRNA delivery reagent which allows very good gene knock-down even with low doses of siRNA. The indicated concentrations of siRNA, directed against GFP mRNA, were delivered in several cell lines seeded in 24-well plates. 2 µL of RiboCellin for HeLa and CHO cells, 3 µL of RiboCellin for SVEC cells and 4 µL of RiboCellin for MDCK cells were used. Cell fluorescence was monitored 96h post-delivery by cytofluorimetry. RiboCellin does not shown any toxicity or non-specific effects.
Figure 2: CHO-GFP were seeded in a 24-well plate the day before siRNA delivery. Cells were treated with 5 nM of a siRNA targeted against GFP and 2 µL of RiboCellin (left) or with 5 nM of a control siRNA and 2 µL of RiboCellin (right). Cells were observed 4 days post delivery by fluorescence mictroscopy.
Figure 3: A549 Cells were seeded in a 24-well plate the day before siRNA delivery. Cells were treated with 10 nM of a siRNA targeted against GFP and 2 µL of RiboCellin (left) or with 10 nM of a control siRNA and 2 µL of RiboCellin (right). Cells were transfected the day after with 0.5 µg of GFP expressing plasmid DNA and 2 µL of GeneCellin. Cells were observed after 48h by fluorescence microscopy.