GeneCellin HTC Transfection Reagent (5x750 µl)

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GeneCellin HTC Transfection Reagent (5x750 µl)
1 760,00 €

Availability: In stock

SKU : HTC3750

GeneCellin HTC provides
highly effective and reliable delivery of nucleic acids into a large variety of cell types, including many hard-to-transfect
cell lines, stem cells and primary cells. Allowing a superior efficiency with many cell types, this r
OR
Description

Details

£1350 / €1500

Supplier
BioCellChallenge
Cat. no.
HTC3750
Kit size
5x750 µl
Links
Protocol
data
Brochure

Features

  • High efficiency & reproducibility
  • Maximum cell viability
  • Serum compatible
  • Dedicated for Primary cells and Hard-to-transfect cells transfection

Genecellin HTC is the latest transfection reagent from BioCellChallenge. This new formulation is based on polymer which have been especially designed to optimize transfection results on hard-to-transfect cells. GeneCellin HTC provides highly effective and reliable delivery of nucleic acids into a large variety of cell types, including many hard-to-transfect cell lines, stem cells and primary cells. Allowing a superior efficiency with many cell types, this reagent keeps a low cytotoxicity and remarkably reproducible results.












Mouse macrophages primary cells Rat Mesenchymal Stem Cells
Protocol:
DNA transfection protocol is provided for a 24-well plate culture vessel. A table is provided to adapt it to other culture formats

    1. Seed adherent healthy cells the day before transfection in order to obtain 60%-70% confluency on the day of transfection (for suspension cells, Split suspension healthy cells the day before transfection according to Table 1 so they could be in logarithmic growth phase at the time of transfection)
    2. Dilute 1 µg of DNA in 100 µL of serum free medium.
    3. Add 2 µL of GeneCellin HTC to the diluted DNA solution and mix the solution by vortexing during 2-3 seconds.
    4. Incubate 15 minutes at room temperature.
    5. Add the 100 µL of GeneCellin HTC / DNA mixture dropwise onto the cells plated in 1 ml of serum containing culture medium and gently rock the plate to ensure an even distribution of the complexes (do not swirl the plate or the dish).
    6. Incubate at 37°C in a CO2 incubator.
    7. Analyse transgene expression 24-48 hours later.
  • Kit Components
GeneCellin HTC Transfection Reagent 5x750 µl
  • Storage and Handling
Upon receipt, the kit should be stored at 4°C. Stored properly, the kit components should remain stable for 1 year.
Transfection comparison with Mesenchymal Stem Cells.



Figure 1: Mesenchymal Stem Cells have been transfected with GeneCellin HTC reagent. We have obtained 35% of transfected cells after 24 h and 30% after 48 h. The results were compared with two others reagents.






Efficient with many hard-to-transfect cell lines, stem cells and primary cells.




  • Plasmid DNA transfection in a wide variety of cells, including many hard-to-transfect cell lines, stem cells and primary cells.
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