Protein Carbonylation Assay Kit

Supplier
BioQuoChem
Cat. no.
KF-03-008
Kit size
100 tests- 96 w format
Links
Protocol

Features

• Fast and easy processing

• Colorimetric measurement

• Linearity over a broad range of concentrations

Oxidative stress may cause reversible or irreversible changes in proteins. Such changes are meant to modulate protein function (redox regulation) or protect against irreversible damage that causes the inactive proteins to accumulate or become degraded.
Carbonylation, an irreversible oxidative damage, involves the oxidation of side chains of amino acids to aldehydes or ketones. Lysine, arginine, proline, and threonine side-chains can be oxidatively converted to reactive aldehyde or ketone groups causing inactivation, crosslinking or breakdown of proteins1.
Protein carbonylation can be detected and quantified at the global level in proteins and protein mixtures using derivatization of carbonyl groups with hidrazines followed by spectrophotometric measurement. 

Wit this kit, the detection of carbonylated proteins is based on 2,4-dinitrophenylhydrazine (DNPH). This molecule reacts with carbonyl groups leading to the formation of the stable 2,4-dinitrophenylhydrazone (DNP). The DNP can be detected and quantified spectrophotometrycally at a typical absorption spectrum with a maximum at 365-375nm.

• Oxidative stress determination

• Protein Carbonylation determination