BQCell™ BlueCell Viability Assay kit

In stock
SKU
KC-04-002B
370,00 €
Blue Cell Viability Assay Kit offers a simple, rapid, reliable, sensitive, safe and cost-effective measurement of cell viability. The BLUE Cell viability kit detects cell viability by utilizing a blue indicator dye resazurin to measure the metabolic capacity of cells
€370

 

Supplier
BQC
Cat. no.
KC-04-002B
Kit size
100 ml

Features

  • Allows to assess cells health prior, after or during living cells based assays
  • single step method allowing detection of cell viability by utilizing a blue and nonfluorescent dye resazurin, which is converted to a pink and fluorescent dye resorufin: allows for both fluorometric (more sensitive) or colorimetric detection
  • This assay is as sensitive as thymidine assay for detecting cell proliferation: Depending on the cell types, BQCell Blue™ can detect as few as 40 cells with reproducible and sensitive signal.

 
BQCell Blue Cell Viability Assay Kit offers a simple, rapid, reliable, sensitive, safe and cost-effective measurement of cell viability. BQCell Blue detects cell viability by utilizing a blue and nonfluorescent dye resazurin, which is converted to a pink and fluorescent dye resorufin in response to chemical reduction of growth medium resulting from cell growth. Continued cell growth maintains a reduced environment while inhibition of growth maintains an oxidized environment. Reduction related to growth causes the REDOX indicator to change from the oxidized (nonfluorescent, blue) form to the reduced (fluorescent, red) form. The fluorescent signal is monitored using 530-580 (579) nm excitation wavelength and 590 (584) nm emission wavelength. The absorbance is monitored at 570 nm and 600 nm. For optimal result, subtract background OD at 600 nm from OD at 570 nm. The fluorescent and colorimetric signal generated from the assay is proportional to the number of living cells in the sample. The BQCell Blue assay is designed to quantitatively measure the proliferation of various human and animal cell lines, bacteria and fungi.
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