Adenoviral particles

Adenovirus products are the ideal tools for expressing or modifying genes for in vitro and in vivo research. Adenovirus is a popular choice for gene delivery applications primarily because of its large packaging capacity, high transgene expression, and ability to infect most cell types; it is also widely used in cardiovascular, liver, muscle, lung, tumor, stem cell and other fields. Once inside the cell, recombinant adenovirus remains episomal and does not integrate into the host genome.

Advantages:

• High titers.  Titer of recombinant adenovirus particles can be up to 10¹² VP/ml.
• Versatile.  Usable in a broad range of host cell and animals.
• Large packaging capacity.  The packaging capacity of recombinant adenovirus can be up to 5 kb.
• High transduction efficiency.  
• Low toxicity.  Does not integrate into the host genome.
• Safe.  Recombinant adenovirus is replication-incompetent (El (early protein) and/or E3 deletion mutants).

Figure 1. Hela, A549, and Vero cells in 12-well plates were transduced with the indicated amounts of eGFP ultra-purified AdenoFect™ Adenovirus particles (Cat#. AD05-EGFP-EB001, 3.39×10¹²vp/mL, 1.56 μl/well) at MOI 10. The expression of eGFP was visualized with a fluorescence microscope at 15, 24, and 48 hours post-transduction respectively.

Custom Adenovirus

Custom adenovirus production services are available for human, mouse and rat ORF cDNAs. For custom adenovirus packaging service, please contact us at info@labomics.com to get a quote.

Choose from our collection of adenovirus vectors with different promoters and fluorescent markers for your gene expression.