Nitrite / Nitrate Determination 100 tests
Nitrite / Nitrate Determination 100 tests (96 well plate)
Fast and easy method for the detection of nitric oxide formation in biological samples. This method relies on an innovative cofactor technology allowing linearity over a broad range of concentration (over 100 µM) and reducing the handling and assay time
The kit is suitable for different sample types: plasma, serum, saliva, urine, cell lysate, tissue homogenate and culture medium.
In this assay, firstly, the nitrate is reduced to nitrite catalyzed by the nitrate reductase with cofactors and specific compounds to eliminate interferences.
The detection is based on the final product detection (diazonium compound, λmáx=540nm) obtained after nitrite reaction in several steps with sulfanilamide.
| Supplier |
|---|
| BioQuoChem |
| Cat. no. |
| KF-03-010 |
| Kit size |
| 100 tests- 96 w format |
| Links |
Protocol  |
Features
- Fast and easy processing
- Colorimetric measurement
- Linearity over a broad range of concentrations
Fast and easy method for the detection of nitric oxide formation in biological samples. This method relies on an innovative cofactor technology allowing linearity over a broad range of concentration (over 100 µM) and reducing the handling and assay time The kit is suitable for different sample types: plasma, serum, saliva, urine, cell lysate, tissue homogenate and culture medium. In this assay, firstly, the nitrate is reduced to nitrite catalyzed by the nitrate reductase with cofactors and specific compounds to eliminate interferences. The detection is based on the final product detection (diazonium compound, λmáx=540nm) obtained after nitrite reaction in several steps with sulfanilamide.
.| Format | 100 tests - 96 well format |
|---|---|
| Colorimetric reading | OD at 540 nm |
- Nitric oxide quantification
- Nitrosative stress determination
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