5x HOT FIREPol Blend High fidelity Master Mix Ready to Load 10mM MgCl2

Supplier
LabOmics Solis Biodyne
Cat. No
6310505
Pack size
1ml (250rxn)
Links
Data Sheet MSDS

Description:

• 5 x HOT FIREPol® Blend Master Mix Ready to Load is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water), additional compound needed for direct loading onto agarose gel and two tracking dyes (blue and yellow) that allow to monitor progress during electrophoresis.
• HOT FIREPol® Blend Master Mix contains two carefully optimized enzymes – HOT FIREPol® DNA polymerase and a proofreading polymerase. This enzyme blend has both the 5’→ 3’ exonuclease activity as well as the 3’→ 5’ proofreading activity. HOT FIREPol® Blend Master Mix exhibits an increased fidelity (up to five fold) compared to HOT FIREPol® Master Mixes.

• Recommendations: Reaction setup at room temperature is highly recommended for HOT FIREPol® Blend Master Mix. We recommend using 5 x HOT FIREPol® Blend Master Mix Ready to Load in any PCR application that will be visualized by agarose gel electrophoresis and ethidium bromide staining. 5 x HOT FIREPol® Blend Master Mix Ready to Load is not recommended for use in applications where spectro-photometric measurements (absorbance or fluorescence) are necessary because yellow and blue dyes can interfere with these applications.
• Shipping and Storage conditions: Routine storage: -20ºC Shipping and temporary storage for up to 1 month at room temperature or storage for up to 6 months at 2-8ºC has no detrimental effects on the quality of 5 x HOT FIREPol® Blend Master Mix Ready to Load.

• Mix Composition:

• 5 x HOT FIREPol® Blend Master Mix Ready to Load

• HOT FIREPol® DNA polymerase
• Proofreading enzyme
• 5 x Blend Master Mix Buffer
• 10 mM MgCl2

1 x PCR solution – 2 mM MgCl2

• 1 mM dNTPs of each

1 x PCR solution – 200 μM dATP, 200 μM dCTP, 200 μM dGTP and 200 μM dTTP

• BSA
• Blue dye

Migration equivalent to 3.5-4.5 kb DNA fragment

• Yellow dye

Migration rate in excess of primers in 1% agarose gel:

• Compound that increases sample density for direct loading

• Hot Start PCR