||For lentiviral and mammalian vector based shRNAs, your set will contain 4 shRNA expression constructs. For validated human kinome shRNA clones in psi-sH1 vector, we provide 1 to 3 clones for each gene, all of which have been validated to have a KD effect of 70% of the original level using colorimetric alkaline phosphatase assay
||The hairpin consists of a 7 base loop and 19~29 base stem optimized for the specific gene sequence.
||The expression cassettes of all shRNA clones are fully sequenced, which includes the promoter, sense and antisense target sequences, hairpin, termination and other linker sequences. The sequences of 19~29mer shRNAi will be delivered with shRNA clones.