AccuTarget Luciferase siRNA
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AccuTarget Positive control Luciferase siRNA |
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Features
- Controls offered at 10, 20, 50 and 100 nmole.
- AccuTargetTM Positive siRNAs can induce effective knockdown of target genes. As an endogenous gene, we offer a positive control siRNA targeting GAPDH and as a reporter system, positive control siRNAs targeting GFP and luciferase genes are available.
Hela cells in a 6-well plate were cotransfected with 400 ng of CMV-luc plasmid and 10 nM of luciferase siRNA using lipofectamine 2000 transfection reagent. Next day, cells were harvested and assayed for luciferase activity. As shown in Fig. 14, cotransfection with our positive control luciferase siRNA led to efficient knockdown of luciferase activity (85% - 95% knockdown compared to luciferase activity of NC-siRNA-transfected cells).
Control siRNA, BioRP purification | 1 nmole: 60 euros | 5 nmole: 85 euros | 10 nmole: 160 euros | 20 nmole: 195 euros | 50 nmole: 295 euros |
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Control siRNA, HPLC purification | 1 nmole: 90 euros | 5 nmole: 160 euros | 10 nmole: 210 euros | 20 nmole: 295 euros | 50 nmole: 370 euros |
Control set (5 nmole (+), 2 nmole(-), BioRP purification | 160 euros | ||||
Control set (5 nmole (+), 2 nmole(-), HPLC purification | 198 euros | ||||
Fluorescein Labeled Negative, HPLC purification | 1 nmole: 98 euros | 5 nmole: 188 euros | 10 nmole: 280 euros | 20 nmole: 365 euros | 50 nmole: 440 euros |
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