AccuTarget GFP siRNA
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AccuTarget Positive control GFP siRNA |
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Bioneer Bioneer information Product information |
Features
- Controls offered at 10, 20, 50 and 100 nmole.
- AccuTargetTM Positive siRNAs can induce effective knockdown of target genes. As an endogenous gene, we offer a positive control siRNA targeting GAPDH and as a reporter system, positive control siRNAs targeting GFP and luciferase genes are available.
Hela cells in a 24-well plate were cotransfected with 200 ng of CMV-GFP plasmid and 10 nM of GFP siRNA using lipofectamine 2000 transfection reagent. Next day, the expression of GFP was observed by using a Nikon Eclipse TS100 epifluorescence microscope. In contrast to bright green fluorescence of GFP protein in NC-siRNA-transfected cells, no fluorescence was detected from GFP-siRNA-transfected cells, indicating efficient knockdown of GFP by using our positive control GFP-siRNA.
Control siRNA, BioRP purification | 1 nmole: 60 euros | 5 nmole: 85 euros | 10 nmole: 160 euros | 20 nmole: 195 euros | 50 nmole: 295 euros |
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Control siRNA, HPLC purification | 1 nmole: 90 euros | 5 nmole: 160 euros | 10 nmole: 210 euros | 20 nmole: 295 euros | 50 nmole: 370 euros |
Control set (5 nmole (+), 2 nmole(-), BioRP purification | 160 euros | ||||
Control set (5 nmole (+), 2 nmole(-), HPLC purification | 198 euros | ||||
Fluorescein Labeled Negative, HPLC purification | 1 nmole: 98 euros | 5 nmole: 188 euros | 10 nmole: 280 euros | 20 nmole: 365 euros | 50 nmole: 440 euros |
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