ORFEXPRESS Gateway® PLUS Shuttle clones

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Gateway® PLUS Shuttle Clones
0,00 €
ORFEXPRESS Gateway® PLUS Shuttle clones offer both recombination and multiple cloning sites making them highly multifunctional and cost-effective. The collection consists of 20,000 human and 15,000 mouse Gateway PLUS shuttle clones.
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GeneCopoeia
Product
ORFEXPRESS Gateway® PLUS Shuttle Clones
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GeneCopoeia
GeneCopoeia information

Features

  • A high value choice for both traditional and recombination cloning: ORFEXPRESS Gateway® PLUS Shuttle clones offer both recombination and multiple cloning sites making them highly multifunctional and cost-effective.


  • Available with and without stop codons


  • Ribosomal binding sites (Shine Dalgarno and Kozak Sequence) for optimal translational context


  • Multiple cloning sites and attL1 and attL2 recombination sites included


  • More than 20,000 human and 15,000 mouse genes available


  • Validated high quality full length protein coding ORF cDNA clones (from start to stop codons)



ORFEXPRESS Gateway® PLUS Shuttle clones offer both recombination and multiple cloning sites making them highly multifunctional and cost-effective. The collection consists of 20,000 human and 15,000 mouse Gateway PLUS shuttle clones.

Compatibility of ORFEXPRESS™ Gateway PLUS shuttle clones with Invitrogen Gateway cloning technology makes the transfer of protein-coding ORFs into any Gateway destination expression vector simple and rapid. These clones can then be used for expression and functional analysis of target proteins in E.coli, yeast, baculovirus, CHO and mammalian cell lines as well as cell-free transcription and translation coupling systems.



Figure1: Gateway® PLUS shuttle ORF cDNA clones can be used for both recombination as well as traditional restriction digestion ligation cloning.


Gateway PLUS provides more options:

Recombination cloning – The presence of attL1 and attL2 sites enables transfer via recombination of ORFs into any destination vector using Gateway technology.

More compatibility - Unlike other Gateway clones, Gateway PLUS enable traditional restriction digestion ligation cloning. The full ORF of Gateway PLUS shuttle clones have multiple cloning sites in the linker between attachment site (attL1 site) and start codon (ATG). This makes them compatible with many other cloning systems using the classical restriction-ligation cloning method.

Quality Assurance: Human and mouse Gateway PLUS shuttle clones are derived from full-length human and mouse genes that were chosen through a strict selection process. This process includes the extraction, comparison as well as validation of gene sequences and annotation information from multiple public and private sources; clustering and reduction of redundant gene sequences, filtering out error sequences or sequencing errors plus several other manual curation steps.

Following this stringent selection process, the entire coding open reading frames (ORFs) were generated from sequence validated full-length cDNA clones or high-quality human tissue cDNA libraries and then inserted into modified Gateway® entry vector using GeneCopoeia's proprietary high – fidelity cloning technologies.

Once the clone is constructed, additional quality control steps are followed to ensure the right clone is delivered to you.

  • All ORFs are fully sequenced.


  • PCR amplification and size validation


  • Enzyme digestion check of the integrity of whole plasmid.


1. Identify the gene of interest:
Please gather as much as possible of the following information: Gene Description, species, Nucleotide Accession, Entrez Gene ID, Symbols/Aliases or Catalog/product ID. You can also submit a sequence so we can search by target gene sequences homology (BLAST)

Alternatively, you can use the search tool by GeneCoppoeia - Search ORF clone by GeneCopoeia - and order the product ID result.


2. Fill out the order form:

and fill with requested information. Send it back to us by email or FAX. We will contact you shortly to confirm: sequences, vectors and controls


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