RNA/microRNA/DNA/Protein Purification Kit

RNA/microRNA/DNA/Protein Purification Kit
294,00 €

Availability: In stock

SKU : 1024200

The All-in-One Purification Kit provides a rapid method for the isolation and purification of total RNA including microRNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast,
Description

Details

Supplier
Norgen Biotek
Cat. no.
1024200
Kit size
20
Links
Norgen Biotek
Protocol
Information Sheet

Features

  • Fast and easy processing using rapid spin-column format
  • All columns for RNA, DNA and protein purification provided
  • Sequentially isolate nucleic acids and proteins from a single lysate – no need to split the lysate
  • Isolate either total RNA, from large rRNA down to microRNA (miRNA) OR separately isolate large RNA species and small RNA species including microRNA
  • No phenol or chloroform extractions
  • Isolate high quality RNA
  • Isolate high quality genomic DNA with a molecular weight = 30 kb
  • High yields of isolated proteins

Norgen’s All-in-One Purification Kit provides a rapid method for the isolation and purification of total RNA including microRNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants. In addition, a microRNA Enrichment Column is provided for the optional separate purification of small RNA(under 200 nt), allowing for 4-in-1 purification (large RNA, genomic DNA, microRNA and proteins). This kit is an ideal all-in-one solution for researchers studying systems biology, including those who are interested in the interactions of multiple disciplines including RNA interference, genomics, epigenomics, transcriptomics and proteomics. Norgen’s All-in-One Purification Kit is especially useful for researchers who are isolating macromolecules from precious, difficult to obtain or small samples such as needle biopsies and single foci from cell cultures, as well as for mutant analysis, RNA interference studies and pathogen detection. Furthermore, analysis will be more reliable since the RNA, DNA and proteins are derived from the same sample without any fractionation, thereby eliminating inconsistent results. The purification procedure is very rapid, allowing for the isolation of large RNA, genomic DNA, microRNA and proteins from a single sample in less than 40 minutes. The isolated macromolecules are of the highest purity and can be used in a number of different downstream applications.
Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. Two different spin columns are provided, and the user can choose whether they wish to isolate total RNA (including microRNA), genomic DNA and total proteins or if they wish to separately isolate microRNA (under 200 nt), large RNA (>200 nt), genomic DNA and proteins. In both cases all the macromolecules are column purified without the use of phenol, chloroform or acetone. The purified molecules are of the highest quality and are well suited for many downstream applications including PCR amplifications, RT-PCR and Real-Time PCR, as well as Southern blots, Northern blots, Western blots, and Dot blots, cDNA synthesis, microarrays and mass spectrometry.
Column Binding capacity (RNA) 50 µg
Column Binding capacity (DNA) 20 µg
Column Binding capacity (Protein) 200 µg
Size of DNA purified >30 kbp
Time to complete 10 purifications 20 minutes
Average RNA yield HeLa cells (1x10exp6 cells): 15 µg RNA HeLa cells (1x10exp6 cells): 8 µg DNA HeLa cells (1x10exp6 cells): 150 µg Protein
Sequential Isolation of microRNA, Large RNA, Genomic DNA and Proteins from 1 x 10 6 HeLa Cells.
Panels A and B show the separate isolation of large RNA and microRNA from 2 different samples of HeLa cells. Panel A is a 1X MOPS 1% agarose gel and Panel B is a 10% urea-PAGE gel. In both gels, Lane M is Norgen’s 1Kb RNA Ladder, Lanes 1 and 2 contain 3 uL out of the 50 uL elutions of the large RNA fraction, and Lanes 3 and 4 contain 3 uL out of the 50 uL elutions of the microRNA fractions. Panel C is a 1% agarose gel showing the gDNA isolated from the same 2 HeLa cell samples. Lane M is Norgen’s UltraRanger DNA Ladder and Lanes 1 and 2 contain 10 uL of each of the 100 uL elutions. Panel D is a 12% SDS-PAGE gel that contains the proteins that were isolated from the 2 HeLa cell samples. Lane M is a protein ladder and Lanes 1 and 2 contain 10 uL of the 100 uL elution of proteins that had been column purified. The large RNA, microRNA, gDNA and proteins are all intact and of the highest integrity and quality. Furthermore, the kit allows for the successful separate isolation of microRNA (under 200 nt) and large RNA (>200nt).

Isolate High Quality RNA.
RNA was isolated from HeLa cells using Norgen’s All-in-One Purification Kit and a leading market competitor for multiple analyte isolations. The purified RNA was used as the template in a qPCR reaction using GAPDH primers, and the results are shown in the PCR baseline graph to the left. The blue lines correspond to the PCR results when RNA isolated using Norgen’s kit was used as the template, while the green lines correspond to the results when RNA isolated using the competitors kit was used as the template. From the graph it can be seen that Norgen’s kit isolated RNA with a greater sensitivity, and that more RNA was isolated from the same input as evidenced by the lower CT values for the Norgen-isolated RNA.


  • PCR amplifications
  • RT-PCR and Real-Time PCR
  • Northern blotting
  • Southern blotting
  • Western blotting
  • Dot blots
  • cDNA synthesis
  • microarrays
  • mass spectrometry
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