ProteoSpin Endotoxin Removal Maxi Kit

Supplier
Norgen Biotek
Cat. no.
1022200
Kit size
24
Links
Norgen Biotek Protocol Information Sheet

Features

• Endotoxin-free proteins and peptides - ProteoSpin™ Endotoxin Removal kits reduce endotoxin levels to less than 0.01 EU/µg of protein.
• Fast and easy processing - Efficient processing of multiple samples in 20 to 30 minutes using an easy-to-use protocol.
• High Recovery - Recovery is over 95% of input.
• Recovered protein is suitable for downstream applications - Purified protein is suitable for in vivo and in vitro introduction into cells and organisms, monoclonal and polyclonal antibody generation.

The ProteoSpin™ Endotoxin Removal Maxi Kit is designed for the rapid removal of endotoxins from up to 4 mg of previously purified proteins or peptides. Endotoxins, also known as lipopolysaccharides, are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins liberated by Gram-negative bacteria are frequent contaminations of protein solutions derived from bioprocesses. Due to the high toxicity of endotoxins in vivo and in vitro, their removal from protein preparations is often necessary prior to the use of the protein in downstream applications.

The ProteoSpin™ Endotoxin Removal Maxi Kit efficiently reduces endotoxin levels to = 0.1 EU/µg of protein using spin column chromatography based on Norgen’s proprietary resin. The purified protein samples can be used in a number of downstream applications including in vitro and in vivo introduction into cells and organisms, sequencing, and cloning. The ProteoSpin™ Endotoxin Removal Maxi Kit contains sufficient materials for 4 purifications. Each column is able to remove endotoxins from up to 4 mg of previously purified proteins, with protein recoveries of > 95% being achieved. Preparation time for a single sample is approximately 30 minutes.

Maximum Protein Input	4 mg
Maximum Protein Volume Input	18 mL
Final Endotoxin Levels	< 0.01 EU/µg protein
Average Recovery	>95 %
Time to Complete 10 Purifications	30 minutes

Endotoxins were removed from 100 µg protein samples in triplicate using the ProteoSpin ™ Endotoxin Removal Micro Kit.

On average, the endotoxin levels in the 100 µg samples were reduced from 0.9 EU/ µg to 0.005 EU/ µg.

Average protein recoveries of greater then 95% were achieved when endotoxins were removed from 100 µg inputs of protein in triplicate.

User-Friendly Maxi Kit Procedure

• in vivo and in vitro introduction into cells and organisms
• monoclonal and polyclonal antibody generation