ProteoSpin Urine Protein Concentration Maxi Kit

In stock
SKU
1021600
168,00 €
ProteoSpin Urine Protein Concentration Maxi Kit
Supplier
Norgen Biotek
Cat. no.
1021600
Kit size
4
Links
Norgen Biotek
Protocol
Information Sheet
MSDS

Features

  • Fast and easy processing - Efficiently process 4 samples in approximately 45 minutes using an easy-to-use protocol.
  • Complete Kit - All columns and solutions required are provided.
  • No molecular weight cutoff - Based on an ion exchange mechanism, a broad size range of proteins can be processed.
  • Proteins bind while salts are discarded in flowthrough - Norgen's resin has an inherent low affinity for salt, but high affinity for proteins, providing salt removal and buffer exchange simultaneously.
  • Suitable for downstream applications - Final elution suitable for: SDS-PAGE, 2D gel electrophoresis, Whole protein mass spectrometry, Protein microarrays.

The ProteoSpin™ Urine Protein Concentration Maxi Kit provides a fast and simple procedure for concentrating dilute solutions of urine proteins from large volumes of urine. Urine protein analysis can be used for the identification of potential biomarkers in urine, and to diagnose and/or monitor renal and other diseases. The purified urine proteins are both concentrated and free of salts, making this kit a convenient method for preparing proteins before running downstream proteomic applications including SDS-PAGE, 2D gels, whole protein mass spectrometry, and protein microarrays.
Purification is based on spin column chromatography using Norgen’s proprietary resin as an ion exchanger. The resin has a poor affinity for monovalent and divalent cations, also making it an effective resin for the removal of salts. Thus in this case, urine proteins are preferentially purified from all other urine components including salts and other wastes. The process involves first collecting the urine sample, and adjusting the pH to 3.5 using the provided pH Binding Buffer (please see flow chart on page 3). By adjusting the pH to 3.5, the urine proteins present will be able to bind to the resin. The maxi spin column is then activated, and the pH-adjusted urine sample is applied to the column. The urine proteins present will bind to the column based on their net charge, while the salts and other wastes will be removed in the flowthrough. The column with the bound proteins is then washed twice with the provided wash buffer, in order to remove any remaining impurities. The purified urine proteins are then eluted in a small volume of the provided elution buffer. The concentrated and salt-free urine proteins can then be used in a number of downstream applications.
Maximum Urine Volume Input 20 mL
Maximum Recovered Protein 4 mg
Minimum Elution Volume 2 mL
Time to Process 4 Samples 45 minutes
No Loss of Urine Proteins
No urine proteins are lost during the binding step, as can be seen by examining the binding flowthrough. Lane A is 25 µL of input urine, Lane B is the binding flowthrough, and Lane C is 15 µL of the elution.


Concentration of Urine Proteins
Urine proteins are concentrated many-fold using the ProteoSpin Urine Protein Concentration Maxi Kit. Lane A contains 20 uL of a urine sample that was used as the input, while Lane B contains 5 uL of the elution, Lane C contains 10 uL of the elution, and Lane D contains 20 uL of the elution . By comparing lanes A and D (equal volume amounts of input and output), it can clearly be seen that the urine proteins have been highly concentrated though the use of the kit.


  • SDS-PAGE
  • 2D gel electrophoresis
  • Whole protein mass spectrometry
  • Protein microarrays
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