ProteoSpin Inclusion Body Isolation Micro Kit (20 preps)

In stock
SKU
1010300
289,00 €
ProteoSpin Inclusion Body Isolation Micro Kit (20 preps)
Supplier
Norgen Biotek
Cat. no.
1010300
Kit size
20
Links
Norgen Biotek
Protocol
Information Sheet
MSDS
Application Notes 1
Application Notes 2

Features

  • Three kits in one for acidic and basic proteins - The kit provides essential reagents for cell disruption, inclusion body solubilization and purification using spin column chromatography. The kit includes solutions and protocols for use with either acidic or basic proteins.
  • Gentle disruption procedure provides high purity final product - Cell lysis is accomplished through non-ionic detergent chemical disruption. The use of a needle and syringe reduces viscosity and facilitates purification, producing protein often >95% pure. Proteins are then ready for SDS-PAGE, 2D gels, mass spectrometry analysis or other procedures.
  • Convenient process volume - Analyze 1.5 mL of microbial cell culture yielding 2–50 ug of protein.
  • Long shelf life - The kit uses an enzyme-free process with stable solutions for longer shelf life.
  • Fast process time - Process up to 12 samples in only 60 minutes.

The ProteoSpin™ Inclusion Body Isolation Micro Kit facilitates the screening of E. coli clones that express recombinant proteins in inclusion bodies. The kit includes reagents specially formulated to achieve rapid and high-quality purification of inclusion body proteins using three processes: 1. Lysis of bacterial cells to release inclusion bodies in solid form 2. Solubilization of purified inclusion bodies 3. Purification of the recombinant protein using spin column chromatography With optimized reagents and streamlined processes, the ProteoSpin™ Inclusion Body Isolation Micro Kit significantly reduces time and labour for screening and identifying clonal cell lines that can be used in scale-up production.
The ProteoSpin™ Inclusion Body Isolation Micro Kit employs spin-column chromatography using Norgen’s proprietary resin as an ion-exchanger. Each spin column is able to purify 2 – 50 µg of recombinant proteins from 1.5 mL of culture. The kit is designed to purify both acidic and basic proteins. Each ProteoSpin™ Inclusion Body Protein Isolation Micro Kit provides essential components for use with the provided protocols. Cell Lysis reagent Inclusion Body solubilization reagent Column activation and wash buffer for acidic proteins pH binding buffer for acidic proteins Column activation and wash buffer for basic proteins pH binding buffer for basic proteins Elution buffer Neutralizer ProteoSpin™ micro spin columns Collection tubes (2 mL capacity) Final collection tubes (1.7 mL capacity) Syringes (1 mL capacity) Needles (20 gauge) ProteoSpin™ Inclusion Body Isolation Micro Kit application manual Protocol reference card for both acidic and basic procedures
Maximum Culture Volume 1.5 mL
Minimum Elution Volume 30 µL
Yield from 1.5 mL of Culture Up to 50 µg
Time to Process 12 Samples 60 minutes
Time course study for expression of a 40 kDa protein.
Expression was induced with 0.4 mM IPTG and incubated at 37 °C for three hours. Aliquots were removed at indicated intervals and purified. Proteins made visible by Coomassie Blue staining.











Isolation of Recombinant RNAseA Protein using ProteoSpin Inclusion Body Protein Isolation Kit.
Bacterial clones expressing recombinant RNAseA protein were induced and resulting inclusion body proteins were purified using the Inclusion Body Protein Isolation Kit and analyzed on 12% SDS-PAGE.












Efficient Isolation of Inclusion Body Proteins Using ProteoSpin Inclusion Body Isolation Kit.
Following gene expression, inclusion bodies were extracted, solubilized and proteins were purified. Recovered proteins were analyzed on 12% SDS-PAGE and stained with Coomassie Brilliant Blue R-250. Numbers represent kDa sizes of protein bands.

  • Mass Spectrometry
  • SDS-PAGE
  • Refolding Experiments
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