ProteoSpin Inclusion Body Isolation Maxi Kit

In stock
SKU
1017700
179,00 €
ProteoSpin Inclusion Body Isolation Maxi Kit
Supplier
Norgen Biotek
Cat. no.
1017700
Kit size
4
Links
Norgen Biotek
Protocol
Information Sheet
Application Notes 1
Application Notes 2

Features

  • Three kits in one for acidic and basic proteins - The kit provides essential reagents for cell disruption, inclusion body solubilization and purification using spin column chromatography. The kit includes solutions and protocols for use with either acidic or basic proteins.
  • Gentle disruption procedure provides high purity final product - Cell lysis is accomplished through non-ionic detergent chemical disruption. The use of a needle and syringe reduces viscosity and facilitates purification, producing protein often >95% pure. Proteins are then ready for SDS-PAGE, 2D gels, mass spectrometry analysis or other procedures.
  • Convenient process volume - Analyze 100 mL of microbial cell culture yielding 12 mg of protein.
  • Long shelf life - The kit uses an enzyme-free process with stable solutions for longer shelf life.
  • Fast process time - Process time is about 2 hours for a single sample.

The ProteoSpin™ Inclusion Body Isolation Maxi Kit facilitates the isolation of recombinant proteins in the form of inclusion bodies from E. coli. The kit includes reagents specially formulated to achieve rapid and high-quality purification of inclusion body proteins using three processes: 1. Lysis of bacterial cells to release inclusion bodies in solid form 2. Solubilization of purified inclusion bodies 3. Purification of the recombinant protein using spin column chromatography With optimized reagents and streamlined processes, the ProteoSpin™ Inclusion Body Isolation Maxi Kit significantly reduces time and labour for isolating and purifying proteins of interest in a larger-scale format.
The ProteoSpin™ Inclusion Body Isolation Maxi Kit employs spin-column chromatography using Norgen’s proprietary resin as an ion-exchanger. Each spin column is able to purify up to 12 mg of recombinant proteins from 100 mL of culture. The kit is designed to purify both acidic and basic proteins. Each ProteoSpin™ Inclusion Body Isolation Maxi Kit provides essential components for use with the provided protocols. Cell Lysis reagent Inclusion Body Solubilization Reagent Column Activation and Wash Buffer for Acidic Proteins pH Binding Buffer for Acidic Proteins Column activation and wash buffer for basic proteins pH Binding Buffer for Basic Proteins Elution Buffer Neutralizer ProteoSpin™ Maxi Spin Columns Collection Tubes Elution Tubes Syringes Needles Product Protocol Insert
Maximum Culture Volume 100 mL
Minimum Elution Volume 4 mL
Yield from 100 mL of Culture Up to 12 mg
Time to Process 1 Sample 60 minutes
Time course study for expression of a 40 kDa protein.
Expression was induced with 0.4 mM IPTG and incubated at 37 °C for three hours. Aliquots were removed at indicated intervals and purified. Proteins made visible by Coomassie Blue staining.










Efficient Isolation of Inclusion Body Proteins Using ProteoSpin Inclusion Body Isolation Kit.
Following gene expression, inclusion bodies were extracted, solubilized and proteins were purified. Recovered proteins were analyzed on 12% SDS-PAGE and stained with Coomassie Brilliant Blue R-250. Numbers represent kDa sizes of protein bands.












Isolation of Recombinant RNAseA Protein using ProteoSpin Inclusion Body Protein Isolation Kit.
Bacterial clones expressing recombinant RNAseA protein were induced and resulting inclusion body proteins were purified using the Inclusion Body Protein Isolation Kit and analyzed on 12% SDS-PAGE.

  • Mass Spectrometry
  • SDS-PAGE
  • Refolding Experiments
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