Bacterial Genomic DNA Isolation Kit
Bacterial Genomic DNA Isolation Kit
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Supplier |
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Norgen Biotek |
Cat. no. |
1017900 |
Kit size |
50 |
Links |
Norgen Biotek Protocol Information Sheet |
Features
- Fast and easy processingRapid spin-column format allows for the processing of multiple samples in 1 hour.
- High binding capacity of columns The binding capacity of the columns in the Bacterial Genomic DNA Isolation Kit is 25 µg.
- Isolate genomic DNA from all types of bacteria Genomic DNA can be isolated from both gram negative and gram positive bacteria.
- Recovered genomic DNA is suitable for downstream applications Purified genomic DNA is fully compatible with restriction enzyme digestions, sequencing and PCR analysis.
- High quality DNA No degradation of the genomic DNA isolated with the Bacterial Genomic DNA Isolation Kit is observed.
Norgens Bacterial Genomic DNA Isolation Kit is designed for the rapid preparation of genomic DNA from 2 x 109 viable bacterial cells (between 0.5 and 1 mL of culture). Purification is based on spin column chromatography using Norgens patented resin as the separation matrix. Norgens resin binds DNA under high salt concentrations and releases the bound DNA under low salt and slightly alkali conditions. The purified DNA is free of RNA and cellular proteinaceous components, and is suitable for many downstream applications.
Maximum Input | 2 x 109 bacterial cells |
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Average Yield* | Up to 20 µg |
Column Binding Capacity | 25 µg |
Time to Complete 10 Purifications | 1 hour |
Time to Complete 10 Purifications |
Isolation of Genomic DNA from both Gram Positive and Gram Negative Bacteria
The Bacterial Genomic DNA Isolation Kit was successfully used to isolate genomic DNA from E. coli (gram negative), B. cereus (lysozyme-resistant gram positive) and M. luteus (gram positive) bacteria.High Yield Purification
The high yield of the Bacterial Genomic DNA Isolation Kit is illustrated by purifying genomic DNA from both a gram positive and a gram negative strain, and comparing the yields with two major competitors. With both types of bacteria, Norgens kit was found to give a higher recovery after both 1 and 2 elutions.Write Your Own Review