Bacterial Genomic DNA Isolation Kit

In stock
SKU
1017900
151,00 €
Bacterial Genomic DNA Isolation Kit
{tab=Overview}
Supplier
Norgen Biotek
Cat. no.
1017900
Kit size
50
Links
Norgen Biotek
Protocol
Information Sheet

Features

  • Fast and easy processingRapid spin-column format allows for the processing of multiple samples in 1 hour.
  • High binding capacity of columns – The binding capacity of the columns in the Bacterial Genomic DNA Isolation Kit is 25 µg.
  • Isolate genomic DNA from all types of bacteria – Genomic DNA can be isolated from both gram negative and gram positive bacteria.
  • Recovered genomic DNA is suitable for downstream applications – Purified genomic DNA is fully compatible with restriction enzyme digestions, sequencing and PCR analysis.
  • High quality DNA – No degradation of the genomic DNA isolated with the Bacterial Genomic DNA Isolation Kit is observed.

Norgen’s Bacterial Genomic DNA Isolation Kit is designed for the rapid preparation of genomic DNA from 2 x 109 viable bacterial cells (between 0.5 and 1 mL of culture). Purification is based on spin column chromatography using Norgen’s patented resin as the separation matrix. Norgen’s resin binds DNA under high salt concentrations and releases the bound DNA under low salt and slightly alkali conditions. The purified DNA is free of RNA and cellular proteinaceous components, and is suitable for many downstream applications.
Maximum Input 2 x 109 bacterial cells
Average Yield* Up to 20 µg
Column Binding Capacity 25 µg
Time to Complete 10 Purifications 1 hour
Time to Complete 10 Purifications
Isolation of Genomic DNA from both Gram Positive and Gram Negative Bacteria
The Bacterial Genomic DNA Isolation Kit was successfully used to isolate genomic DNA from E. coli (gram negative), B. cereus (lysozyme-resistant gram positive) and M. luteus (gram positive) bacteria.




High Yield Purification
The high yield of the Bacterial Genomic DNA Isolation Kit is illustrated by purifying genomic DNA from both a gram positive and a gram negative strain, and comparing the yields with two major competitors. With both types of bacteria, Norgen’s kit was found to give a higher recovery after both 1 and 2 elutions.


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