5x HOT FIREPol EvaGreen® qPCR Mix Plus (no ROX)

In stock
SKU
6345000
1 280,00 €
optimised ready-to-use solution for real-time quantitative PCR assays, incorporating EvaGreen® dye (same usage but higher performances than Sybr green)
£1.174/ €1.280

Supplier
LabOmics Solis Biodyne
Cat. No
6345000
Pack size
20ml (5000rxn)
Links
Data Sheet
MSDS

Features

  • much less PCR inhibition compare to SYBR® Green
  • extremely stable dye
  • nonmutagenic and noncytotoxic

HOT FIREPol® EvaGreen® qPCR Mix Plus(no ROX) is an optimised ready-to-use solution for real-time quantitative PCR assays, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2 and EvaGreen® dye. The user simply needs to add water, template and primers. HOT FIREPol® DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of nonspecifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
  • EvaGreen® Dye: EvaGreen® is a DNA-binding dye with many features that make it a superior alternative to SYBR® Green I for qPCR. Apart from having similar spectra, EvaGreen® has three important features that set it apart from SYBR® Green I: EvaGreen® has much less PCR inhibition, is extremely stable dye and has been shown to be nonmutagenic and noncytotoxic. EvaGreen® is compatible with all common real-time PCR cyclers – simply select the standard settings for SYBR® Green or FAM!
  • Shipping and Storage conditions: Routine storage: -20ºC Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol® EvaGreen® qPCR Mix Plus (no ROX).
  • Mix Composition:
    • HOT FIREPol® DNA Polymerase
    • 5 x EvaGreen® qPCR buffer
    • 12.5 mM MgCl2
1 x PCR solution – 2.5 mM MgCl2
  • dNTPs, including dTTP to improve reaction sensitivity and efficiency compared to dUTP
  • EvaGreen® dye
  • No ROX dye
Log scale amplification plot of FBA1 gene.
Amplification of a 92 bp fragment from FBA1 gene was performed on serial fourfold dilutions of Saccharomyces cerevisiae genomic DNA. Reactions were performed on an Applied Biosystems 7900HT Real-Time PCR System and ROX was used as a passive reference for normalisation of the reporter signal.

  • Detection and quantification of DNA and cDNA targets
  • Profiling gene expression
  • Microbial detection
  • Viral load determination
  • High Resolution Melt (HRM) Analysis
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