Firepol (regular Taq DNA Polymerase) 500 U

In stock
SKU
6100500
29,00 €
FIREPol® is a highly processive, thermostable DNA polymerase.
£27 / €29

Supplier
LabOmics Solis Biodyne
Cat. No
6100500
Pack size
500 units
Links
Data Sheet
MSDS

Features

  • enhanced stability at room temperature: no activity loss for up to 1 month at RT
  • Shipped at RT with no activity loss: save on shipping costs
  • highly processive, thermostable DNA polymerase: 5’→3’ polymerization-dependent exonuclease replacement activity but lacks 3’→ 5’ exonuclease activity.

FIREPol® is a highly processive, thermostable DNA polymerase. Due to its genetic modifications FIREPol® has an enhanced stability at room temperature with no activity loss for up to 1 month. The enzyme has 5’→3’ polymerization-dependent exonuclease replacement activity but lacks 3’→ 5’ exonuclease activity.
  • Source: Purified from an E.coli strain that carries an overproducing plasmid containing a modified gene of Thermus aquaticus DNA Polymerase.
  • Concentration: 5U/µL
  • Unit definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTPs into an acid-insoluble form in 30 minutes at 74ºC.
  • Storage and Dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers.
  • Quality control: The enzyme is free of nicking and priming activities, exonucleases and non-specific endonucleases. SDS/PAGE - 95 kD band, >98% pure. Activity and stability tested via thermo-cycling. The error rate per nucleotide per cycle is ~ 2.5 x 10-5; the accuracy is ~ 4 x 104. Estimated half life at 95ºC is 1.5 hours.
  • Shipping and Storage conditions: Routine storage: -20ºC Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of FIREPol® DNA Polymerase.
  • Mix Composition:
    • FIREPol® DNA Polymerase
    • 10 x Reaction buffer B (Mg2+ free)
0.8 M Tris-HCl, 0.2 M (NH4)2SO4, 0.2% w/v Tween-20
    • 10 x Reaction buffer BD(Mg2+ and detergent free)
0.8 M Tris-HCl, 0.2 M (NH4)2SO4
    • 25 mM MgCl2
    • 10 x Solution S
Additive that facilitates amplification of difficult templates (e.g. GC-rich DNA templates). This solution should be used at a defined working concentration (1x, 2x or 3x solution). Solution S is NOT a reaction buffer and should be used ONLY IF non-specific amplifications occur.
  • Suited for a wide range of PCR assays
  • TA cloning
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