All-in-One™ miRNA qRT-PCR Detection Kit and validated miRNA primers

All-in-One™ miRNA qRT-PCR Detection Kit and validated miRNA primers
0,00 €

Availability: In stock

SKU : MicroRNA analysis RT-qPCR

miRNA PCR analysis detection and profiling through RT-qPCR system
validated miRNA primers
Description

Details

Supplier
GeneCopoeia
Product
All-in-One™ miRNA qRT-PCR Detection Kit
and validated miRNA primers
Links
GeneCopoeia
GeneCopoeia information

Features

  • Sensitive and practical quantitative profiling of miRNA expression


  • Detects only mature miRNA


  • Two-step detection and quantification method of multiple miRNAs from one cDNA synthesis.


  • Combines PCR technology and SYBR® Green: fast and accurate quantitative analysis of mature human miRNAs from total RNA samples as small as 100 pg


  • Novel optimized blend of poly A polymerase and reverse transcriptase with an RT buffer specifically developed for the All-in-One Reverse Transcriptase Mix: delivers maximum activity of both enzymes for robust and reproducible data.


  • 700 validated miRNA primers available







Fig. 1. Overview of steps involved in the All-in-One miRNA qRT-PCR Detection Kit. During 3' polyadenylation, M-MLV RTase and a unique Oligo dT Adaptor primer, reverse transcribes the poly A miRNAs. The kit qRT-PCR Mix, containing SYBR® Green specifically detects the reverse transcribed miRNAs.
The All-in-One qPCR miRNA-specific primers are designed by a proprietary algorithm and validated for more than 700 human miRNAs. When used in combination with the All-in-One SYBR® Green miRNA qRT-PCR kit, the All-in-One miRNA primers deliver reliable and reproducible high performance in quantitative PCR assays.







Fig. 2. All-in-One miRNA qPCR pimers are validated to generate a single amplification of the correct size for the targeted miRNA and to yield a single dissociation curve peak. A cDNA pool, containing reverse transcribed products from 10 human tissue total RNA samples was used as the validation template. qPCR was performed using 0.2 uM primer with 2X All-in-One qPCR Mix. (2A) validated result for amplification curve (2B) validated result for melting curve.
  • high-performance gene-expression profiling human miRNAs.
  • Combine with our validated mature miRNA-specific primers: more than 700 human miRNA primers.
  • Design experiments with our precursor miRNA plasmid constructs and assays for comprehensive functional analysis studies.
  • Follow-up with functional validation studies using our ORF expression-ready clones.
  • See also:
1. Cat No AOMD-Q020, All-in-One miRNA qRT-PCR Detection Kit (20 RT and 200 qPCR reactions): 395 €
Poly A Polymerase, RTase Mix, qPCR Mix, ROX Reference Dye, Universal Adaptor PCR Primer and other buffers; miRNA-specific primers sold separately.

2. Cat No AOMD-Q050, All-in-One miRNA qRT-PCR Detection Kit (50 RT and 500 qPCR reactions): 780 €
Poly A Polymerase, RTase Mix, qPCR Mix, ROX Reference Dye, Universal Adaptor PCR Primer and other buffers; miRNA-specific primers sold separately.

3. For validated specific primers (118 €): Identify the miRNA of interest:
You can browse the entire Pimers list
and use the website search tool to find the primer matching the mature miRNA of interest; Or gather as much as possible of the following information: Precursor/Mature miRNA Name, Precursor/Mature miRNA Accession, Description or Catalog/product ID.
You can also submit a sequence so we can search by Precursor or Mature Sequences homology (BLAST) and we will do the rest.

Alternatively, you can use the search tool by GeneCoppoeia - Search miRNA clones and qPCR primers by GeneCopoeia - and order the product ID result.


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