miProfile™ miRNA qPCR Arrays

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miRNA qPCR Arrays
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The miProfile miRNA PCR Arrays are designed for profiling the expressions of pre-defined or customized sets of miRNAs in various tissues or cells. The resulting differential expressions of profiled miRNAs help researchers to identify those miRNAs that are
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GeneCopoeia
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miProfile™ miRNA qPCR Arrays
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Features

    • Genome-wide coverage, pre-arranged groups, or customized groups Largest genome-wide miRNA coverage, Cancer-related groups or Customized miRNA arrays for focused study
    • Sensitive: Detect miRNA from as little as 10 pg of small RNA or 20 pg of total RNA
    • Specific: Be able to distinguish miRNAs with single nucleotide mismatches. Each primer set has been experimental validated for specific amplification
    • Broad linearity: Allow miRNA at varieties of expression level to be detected simultaneously
    • Reproducible: High reproducibility (R2>0.99) for inter-array and intra-array replicates
    • Validated miRNA primers: Each miRNA primer is designed using a proprietary algorithm and experimentally validated


MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at the post-transcriptional level. Usually 21-23 nucleotides in length, miRNAs are important modulators in cellular pathways and are highly conserved in eukaryotic organisms. Irregularities in miRNA-regulated gene expression have been found to be associated with cancers, cardiovascular disorders and a variety of other diseases. The miProfile miRNA PCR Arrays are designed for profiling the expressions of pre-defined or customized sets of miRNAs in various tissues or cells. The resulting differential expressions of profiled miRNAs help researchers to identify those miRNAs that are of biological significance and importance relevant to their research. Each 96-well plate contains up to 84 pairs of PCR primers (forward: miRNA-specific primer; reverse: universal primer), which have been pre-validated and deposited in designated wells. Each plate also has 12 wells that contain different types of controls for monitoring the efficiency of the entire experimental process: from reverse transcription to qPCR reaction. Using a universal real-time PCR condition, one can easily profile and analyze the miRNA expression in a high-throughput fashion.The All-in-One™ miRNA First-Strand cDNA Synthesis Kits and qPCR Mix Kits are the recommended and supported RT-PCR reagents for use with the miProfile miRNA qPCR arrays. These reagents have been optimized to produce high sensitivity, efficiency, and specificity.


miRNome qPCR arrays

Cat. NoDescriptionNumber of miRNAsPriceDatasheet Primer list
PAM-HG96 miProfile™ human miRNome miRNA qPCR arrays 1,565 (miRBase V.17) - 19 x 96-well plates 2 sets: 1750 € / 4 sets: 2295 € List & Layout
PAM-HS96 miProfile™ human single-nucleotide mismatch miRNA qPCR arrays 61 - 1 x 96-well plate 139 € (2 sets) List & Layout


Cancer miRNA qPCR arrays

Cat. NoDescriptionNumber of miRNAsPriceDatasheet Primer list
PAM-HC96 miProfile™ human cancer miRNA qPCR arrays 420 - 5 x 96-well plates 2 sets: 1295 € / 4 sets: 1850 € / 12 sets: 3850 € List & Layout
PAM-HCN96 miProfile™ human brain cancer miRNA qPCR arrays 84 - 1 x 96-well plate 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € List & Layout
PAM-HCB96 miProfile™ human breast cancer miRNA qPCR arrays 168 - 2 x 96-well plates 2 sets: 535 € / 4 sets: 790 € / 12 sets: 1690 € List & Layout
PAM-HCX96 miProfile™ human leukemia miRNA qPCR arrays 168 - 2 x 96-well plates 2 sets: 535 € / 4 sets: 790 € / 12 sets: 1690 € List & Layout
PAM-HCL96 miProfile™ human lung cancer miRNA qPCR arrays 168 - 2 x 96-well plates 2 sets: 535 € / 4 sets: 790 € / 12 sets: 1690 € List & Layout
PAM-HCO96 miProfile™ human ovarian cancer miRNA qPCR arrays 168 - 2 x 96-well plates 2 sets: 535 € / 4 sets: 790 € / 12 sets: 1690 € List & Layout
PAM-HCY96 miProfile™ human bladder cancer miRNA qPCR arrays 79 - 1 x 96-well plate 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € List & Layout
PAM-HCC96 miProfile™ human colorectal cancer miRNA qPCR arrays 84 - 1 x 96-well plate 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € List & Layout
PAM-HCE96 miProfile™ human endometrial cancer miRNA qPCR arrays 84 - 1 x 96-well plate 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € List & Layout
PAM-HCG96 miProfile™ human gastric cancer miRNA qPCR arrays 80 - 1 x 96-well plate 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € List & Layout
PAM-HCH96 miProfile™ human hepatocellular carcinoma miRNA qPCR arrays 168 - 2 x 96-well plates 2 sets: 535 € / 4 sets: 790 € / 12 sets: 1690 € List & Layout
PAM-HCZ96 miProfile™ human lymphoma miRNA qPCR arrays 84 - 1 x 96-well plate 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € List & Layout
PAM-HCM96 miProfile™ human melanoma miRNA qPCR arrays 84 - 1 x 96-well plate 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € List & Layout
PAM-HCT96 miProfile™ human head and neck cancer miRNA qPCR arrays 84 - 1 x 96-well plate 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € List & Layout
PAM-HCP96 miProfile™ human pancreatic cancer miRNA qPCR arrays 84 - 1 x 96-well plate 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € List & Layout
PAM-HCQ96 miProfile™ human prostate cancer miRNA qPCR arrays 84 - 1 x 96-well plate 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € List & Layout
PAM-MCB96 miProfile™ mouse breast cancer miRNA qPCR arrays 84 - 1 x 96-well plate 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € List & Layout
PAM-MCN96 miProfile™ mouse brain cancer miRNA qPCR arrays 84 - 1 x 96-well plate 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € List & Layout
PAM-MCO96 miProfile™ mouse ovarian cancer miRNA qPCR arrays 84 - 1 x 96-well plate 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € List & Layout
PAM-MCQ96 miProfile™ mouse prostate cancer miRNA qPCR arrays 84 - 1 x 96-well plate 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € List & Layout


Disease and focus-group miRNA qPCR arrays

Cat. NoDescriptionNumber of miRNAsPriceDatasheet Primer list
PAM-HF96 miProfile™ human inflammatory miRNA qPCR array 84 - 1 x 96-well plate 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € List & Layout
PAM-HH96 miProfile™ human heart disease miRNA qPCR array 84 - 1 x 96-well plate 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € List & Layout
PPAM-HI96 miProfile™ human immunopathology miRNA qPCR array 84 - 1 x 96-well plate 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € List & Layout
PAM-HK96 miProfile™ human IPS (Stem cell) miRNA qPCR arrays 168 - 2 x 96-well plates 2 sets: 535 € / 4 sets: 790 € / 12 sets: 1690 € List & Layout
PAM-HM96 miProfile™ human muscle disease miRNA qPCR array 84 - 1 x 96-well plate 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € List & Layout
PAM-HT96 miProfile™ human toxicology related miRNA qPCR array 84 - 1 x 96-well plate 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € List & Layout
PAM-HX96 miProfile™ human serum and plasma miRNA qPCR arrays 168 - 2 x 96-well plates 2 sets: 535 € / 4 sets: 790 € / 12 sets: 1690 € List & Layout
PAM-MF96 miProfile™ mouse inflammatory miRNA qPCR arrays 84 - 1 x 96-well plate 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € List & Layout
PAM-MH96 miProfile™ mouse heart disease miRNA qPCR arrays 84 - 1 x 96-well plate 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € List & Layout
PAM-MI96 miProfile™ mouse immunopathology miRNA qPCR arrays 84 - 1 x 96-well plate 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € List & Layout
PAM-MX96 miProfile™ mouse serum and plasma miRNA qPCR arrays 84 - 1 x 96-well plate 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € List & Layout


miRNA qPCR array custom services

Cat. NoDescriptionNumber of miRNAsPriceDatasheet Primer list
PAM-CS96 miProfile™ miRNA qPCR arrays custom services Variable on request Variable


Protocol overview























Fig. 1. miRNA PCR array experimental work flow (left) and miRNA RT-PCR mechanism (right).
Linear Range and Sensitivity (Total RNA):






















Fig. 2. Starting with serially diluted amount of human colon cancer total RNA, miR-21 and miR-1260 were detected using All-in-One™ miRNA qRT-PCR Detection Kit . The resulting Ct values were plotted against the log10 of the amount of input total RNA. The data demonstrated a broad linear dynamic range from 20 pg to 2 µg of input total RNA as well as high sensitivity. This allows varieties of expression levels of miRNA, including the low abundant ones, to be detected simultaneously.



Inter-Array Reproducibility:




























Fig. 3. Two miProfile PCR array replicates (plate A and B) were analyzed using human total RNA (10-tissue mix) on the Bio-Rad iQ5. The Ct values of the replicate plates were plotted against each other. R2 > 0.99 were observed for high inter-array reproducibility. R2 > 0.99 is also observed for intra-array reproducibility (data not shown).



Single Nucleotide Mismatches:

Fig. 4. Specificity of miRNA detection miRNA miR-29a and miR-29c with one single nucleotide mismatch (B) can be distinguished. Relative detection, defined as a percentage of the perfect match (100% x 2 -?Ct), was calculated using the Ct values of on-target and off-target assays, which were performed to detect miRNA plasmid DNA templates using All-in-One miRNA qRT-PCR Detection Kits (A).
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