miProfile™ miRNA qPCR Arrays
The miProfile miRNA PCR Arrays are designed for profiling the expressions of pre-defined or customized sets of miRNAs in various tissues or cells. The resulting differential expressions of profiled miRNAs help researchers to identify those miRNAs that are
Supplier |
---|
GeneCopoeia |
Product |
miProfile™ miRNA qPCR Arrays |
Links |
GeneCopoeia information |
Features
- Genome-wide coverage, pre-arranged groups, or customized groups Largest genome-wide miRNA coverage, Cancer-related groups or Customized miRNA arrays for focused study
- Sensitive: Detect miRNA from as little as 10 pg of small RNA or 20 pg of total RNA
- Specific: Be able to distinguish miRNAs with single nucleotide mismatches. Each primer set has been experimental validated for specific amplification
- Broad linearity: Allow miRNA at varieties of expression level to be detected simultaneously
- Reproducible: High reproducibility (R2>0.99) for inter-array and intra-array replicates
- Validated miRNA primers: Each miRNA primer is designed using a proprietary algorithm and experimentally validated
MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at the post-transcriptional level. Usually 21-23 nucleotides in length, miRNAs are important modulators in cellular pathways and are highly conserved in eukaryotic organisms. Irregularities in miRNA-regulated gene expression have been found to be associated with cancers, cardiovascular disorders and a variety of other diseases. The miProfile miRNA PCR Arrays are designed for profiling the expressions of pre-defined or customized sets of miRNAs in various tissues or cells. The resulting differential expressions of profiled miRNAs help researchers to identify those miRNAs that are of biological significance and importance relevant to their research. Each 96-well plate contains up to 84 pairs of PCR primers (forward: miRNA-specific primer; reverse: universal primer), which have been pre-validated and deposited in designated wells. Each plate also has 12 wells that contain different types of controls for monitoring the efficiency of the entire experimental process: from reverse transcription to qPCR reaction. Using a universal real-time PCR condition, one can easily profile and analyze the miRNA expression in a high-throughput fashion.The All-in-One™ miRNA First-Strand cDNA Synthesis Kits and qPCR Mix Kits are the recommended and supported RT-PCR reagents for use with the miProfile miRNA qPCR arrays. These reagents have been optimized to produce high sensitivity, efficiency, and specificity.
miRNome qPCR arrays
Cancer miRNA qPCR arrays
Disease and focus-group miRNA qPCR arrays
miRNA qPCR array custom services
Cat. No | Description | Number of miRNAs | Price | Datasheet Primer list |
---|---|---|---|---|
PAM-HG96 | miProfile™ human miRNome miRNA qPCR arrays | 1,565 (miRBase V.17) - 19 x 96-well plates | 2 sets: 1750 € / 4 sets: 2295 € | List & Layout |
PAM-HS96 | miProfile™ human single-nucleotide mismatch miRNA qPCR arrays | 61 - 1 x 96-well plate | 139 € (2 sets) | List & Layout |
Cancer miRNA qPCR arrays
Cat. No | Description | Number of miRNAs | Price | Datasheet Primer list |
---|---|---|---|---|
PAM-HC96 | miProfile™ human cancer miRNA qPCR arrays | 420 - 5 x 96-well plates | 2 sets: 1295 € / 4 sets: 1850 € / 12 sets: 3850 € | List & Layout |
PAM-HCN96 | miProfile™ human brain cancer miRNA qPCR arrays | 84 - 1 x 96-well plate | 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € | List & Layout |
PAM-HCB96 | miProfile™ human breast cancer miRNA qPCR arrays | 168 - 2 x 96-well plates | 2 sets: 535 € / 4 sets: 790 € / 12 sets: 1690 € | List & Layout |
PAM-HCX96 | miProfile™ human leukemia miRNA qPCR arrays | 168 - 2 x 96-well plates | 2 sets: 535 € / 4 sets: 790 € / 12 sets: 1690 € | List & Layout |
PAM-HCL96 | miProfile™ human lung cancer miRNA qPCR arrays | 168 - 2 x 96-well plates | 2 sets: 535 € / 4 sets: 790 € / 12 sets: 1690 € | List & Layout |
PAM-HCO96 | miProfile™ human ovarian cancer miRNA qPCR arrays | 168 - 2 x 96-well plates | 2 sets: 535 € / 4 sets: 790 € / 12 sets: 1690 € | List & Layout |
PAM-HCY96 | miProfile™ human bladder cancer miRNA qPCR arrays | 79 - 1 x 96-well plate | 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € | List & Layout |
PAM-HCC96 | miProfile™ human colorectal cancer miRNA qPCR arrays | 84 - 1 x 96-well plate | 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € | List & Layout |
PAM-HCE96 | miProfile™ human endometrial cancer miRNA qPCR arrays | 84 - 1 x 96-well plate | 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € | List & Layout |
PAM-HCG96 | miProfile™ human gastric cancer miRNA qPCR arrays | 80 - 1 x 96-well plate | 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € | List & Layout |
PAM-HCH96 | miProfile™ human hepatocellular carcinoma miRNA qPCR arrays | 168 - 2 x 96-well plates | 2 sets: 535 € / 4 sets: 790 € / 12 sets: 1690 € | List & Layout |
PAM-HCZ96 | miProfile™ human lymphoma miRNA qPCR arrays | 84 - 1 x 96-well plate | 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € | List & Layout |
PAM-HCM96 | miProfile™ human melanoma miRNA qPCR arrays | 84 - 1 x 96-well plate | 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € | List & Layout |
PAM-HCT96 | miProfile™ human head and neck cancer miRNA qPCR arrays | 84 - 1 x 96-well plate | 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € | List & Layout |
PAM-HCP96 | miProfile™ human pancreatic cancer miRNA qPCR arrays | 84 - 1 x 96-well plate | 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € | List & Layout |
PAM-HCQ96 | miProfile™ human prostate cancer miRNA qPCR arrays | 84 - 1 x 96-well plate | 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € | List & Layout |
PAM-MCB96 | miProfile™ mouse breast cancer miRNA qPCR arrays | 84 - 1 x 96-well plate | 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € | List & Layout |
PAM-MCN96 | miProfile™ mouse brain cancer miRNA qPCR arrays | 84 - 1 x 96-well plate | 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € | List & Layout |
PAM-MCO96 | miProfile™ mouse ovarian cancer miRNA qPCR arrays | 84 - 1 x 96-well plate | 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € | List & Layout |
PAM-MCQ96 | miProfile™ mouse prostate cancer miRNA qPCR arrays | 84 - 1 x 96-well plate | 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € | List & Layout |
Disease and focus-group miRNA qPCR arrays
Cat. No | Description | Number of miRNAs | Price | Datasheet Primer list |
---|---|---|---|---|
PAM-HF96 | miProfile™ human inflammatory miRNA qPCR array | 84 - 1 x 96-well plate | 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € | List & Layout |
PAM-HH96 | miProfile™ human heart disease miRNA qPCR array | 84 - 1 x 96-well plate | 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € | List & Layout |
PPAM-HI96 | miProfile™ human immunopathology miRNA qPCR array | 84 - 1 x 96-well plate | 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € | List & Layout |
PAM-HK96 | miProfile™ human IPS (Stem cell) miRNA qPCR arrays | 168 - 2 x 96-well plates | 2 sets: 535 € / 4 sets: 790 € / 12 sets: 1690 € | List & Layout |
PAM-HM96 | miProfile™ human muscle disease miRNA qPCR array | 84 - 1 x 96-well plate | 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € | List & Layout |
PAM-HT96 | miProfile™ human toxicology related miRNA qPCR array | 84 - 1 x 96-well plate | 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € | List & Layout |
PAM-HX96 | miProfile™ human serum and plasma miRNA qPCR arrays | 168 - 2 x 96-well plates | 2 sets: 535 € / 4 sets: 790 € / 12 sets: 1690 € | List & Layout |
PAM-MF96 | miProfile™ mouse inflammatory miRNA qPCR arrays | 84 - 1 x 96-well plate | 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € | List & Layout |
PAM-MH96 | miProfile™ mouse heart disease miRNA qPCR arrays | 84 - 1 x 96-well plate | 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € | List & Layout |
PAM-MI96 | miProfile™ mouse immunopathology miRNA qPCR arrays | 84 - 1 x 96-well plate | 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € | List & Layout |
PAM-MX96 | miProfile™ mouse serum and plasma miRNA qPCR arrays | 84 - 1 x 96-well plate | 2 sets: 495 € / 4 sets: 750 € / 12 sets: 1650 € | List & Layout |
miRNA qPCR array custom services
Cat. No | Description | Number of miRNAs | Price | Datasheet Primer list |
---|---|---|---|---|
PAM-CS96 | miProfile™ miRNA qPCR arrays custom services | Variable | on request | Variable |
Protocol overview
Fig. 1. miRNA PCR array experimental work flow (left) and miRNA RT-PCR mechanism (right).
Fig. 1. miRNA PCR array experimental work flow (left) and miRNA RT-PCR mechanism (right).
Linear Range and Sensitivity (Total RNA):
Fig. 2. Starting with serially diluted amount of human colon cancer total RNA, miR-21 and miR-1260 were detected using All-in-One™ miRNA qRT-PCR Detection Kit . The resulting Ct values were plotted against the log10 of the amount of input total RNA. The data demonstrated a broad linear dynamic range from 20 pg to 2 µg of input total RNA as well as high sensitivity. This allows varieties of expression levels of miRNA, including the low abundant ones, to be detected simultaneously.
Inter-Array Reproducibility:
Fig. 3. Two miProfile PCR array replicates (plate A and B) were analyzed using human total RNA (10-tissue mix) on the Bio-Rad iQ5. The Ct values of the replicate plates were plotted against each other. R2 > 0.99 were observed for high inter-array reproducibility. R2 > 0.99 is also observed for intra-array reproducibility (data not shown).
Single Nucleotide Mismatches:
Fig. 4. Specificity of miRNA detection miRNA miR-29a and miR-29c with one single nucleotide mismatch (B) can be distinguished. Relative detection, defined as a percentage of the perfect match (100% x 2 -?Ct), was calculated using the Ct values of on-target and off-target assays, which were performed to detect miRNA plasmid DNA templates using All-in-One miRNA qRT-PCR Detection Kits (A).
Fig. 2. Starting with serially diluted amount of human colon cancer total RNA, miR-21 and miR-1260 were detected using All-in-One™ miRNA qRT-PCR Detection Kit . The resulting Ct values were plotted against the log10 of the amount of input total RNA. The data demonstrated a broad linear dynamic range from 20 pg to 2 µg of input total RNA as well as high sensitivity. This allows varieties of expression levels of miRNA, including the low abundant ones, to be detected simultaneously.
Inter-Array Reproducibility:
Fig. 3. Two miProfile PCR array replicates (plate A and B) were analyzed using human total RNA (10-tissue mix) on the Bio-Rad iQ5. The Ct values of the replicate plates were plotted against each other. R2 > 0.99 were observed for high inter-array reproducibility. R2 > 0.99 is also observed for intra-array reproducibility (data not shown).
Single Nucleotide Mismatches:
Fig. 4. Specificity of miRNA detection miRNA miR-29a and miR-29c with one single nucleotide mismatch (B) can be distinguished. Relative detection, defined as a percentage of the perfect match (100% x 2 -?Ct), was calculated using the Ct values of on-target and off-target assays, which were performed to detect miRNA plasmid DNA templates using All-in-One miRNA qRT-PCR Detection Kits (A).
Write Your Own Review