Carboxyl-Adembeads Coupling Kit

In stock
SKU
2002820
240,00 €
Carboxyl-Adembeads are uniform superparamagnetic nanoparticles presenting a polymer core shell structure.Unique size distribution: CVfor specific coupling (up to 25 m2/g) Easy to couple: a complete procedure and buffers available for protein coupling
£222 / €240

Supplier
Ademtech
Cat. No
2002820
Kit size
30 coupling
Links
ademtech
Protocol Carboxyl-adembeads coupling kit.pdf
Information Sheet

Features

  • Unique size distribution: CV
  • Rapid and simple way of coupling : no column or centrifugation steps
  • High COOH density
  • Low non specific binding
  • The small size ensures that all particles do not sediment on standing

Carboxyl-Adembeads are available with different sizes making them ideal for use with a variety of molecules including proteins, antibodies. This Coupling Kit has been created so you can test each of these Carboxyl bead products to find the best one for your application. Carboxyl-Adembeads are monodispersed and super-paramagnetic beads composed of magnetic core encapsulated by a highly crosslinked hydrophilic polymer shell. The surface is activated with carboxylic acid functionality. The hydrophilic surface ensures low non-specific binding, excellent dispersion abilities and easy handling of the beads in a wide range of buffers. Carboxyl-Adembeads are produced under asceptic conditions and are sold in an aqueous suspension containing 0.09% NaN3.
The functional carboxylic acid groups of Carboxyl-Adembeads offer the possibility for many different immobilisation procedures for use with proteins or other ligands via EDC activation for example. We recommend coupling 10-50 µg of ligand per mg of beads. Protocols should be optimised to meet your requirements (e.g. sample volume, concentration of ligand / beads/ EDC).
Kit contents The reagents are provided for at least 10 coupling each of 1 mg of Carboxyl-Adembeads of each size.
Beads - Carboxyl-Adembeads, 200 nm, 1 ml (1%)  - Carboxyl-Adembeads, 300 nm, 1 ml (1%) - Carboxyl-Adembeads, 500 nm, 1 ml (1%)
Buffers - Activation Buffer - EDC - Storage Buffer


Carboxyl-Adembeads are designed to act as solid support for a wide variety of biomagnetic separations and manipulations. Proteins, oligonucleotides or other target specific molecules can be easily covalently coupled directly onto the surface of Adembeads via primary amino groups. Once coupled with ligand, the beads can be added to a cell lysate or other suspensions containing your target molecule. After a short incubation, the beads can be pulled to the side of the test tube by use of a magnetic device allowing aspiration of unbound material. Furthermore, the magnet facilitates washing and concentration of the isolated target.
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