Bio-Adembeads Protein G (5 ml)

In stock
SKU
2004331
590,00 €
Magnetic beads coupled with recombinant protein G for high specificity. Only the Fc binding sites are present.Use: immunoprecipitation and small scale Ig purification. Starting sample can be saliva, plasma, ascites, serum and tissue culture or hybridoma
£525 / €590

Supplier
Ademtech
Cat. No
2004331
Kit size
5 ml
Links
ademtech
Protocol
Information Sheet

Features

  • Convenient for small scale Ig purification and immunoprecipitation
  • perfect spherical shape with an important smooth surface area (10 square meter/g)
  • Starting sample can be saliva, plasma, ascites, serum and tissue culture or hybridoma supernatants.
  • Recombinant form of the protein G without albumin and Fab binding sites. This offers an increasing specific tool without background: reduces co-purification of contaminating proteins. Only the Fc binding sites are present.
  • Magnetic technology: high specificity and convenience (no centrifugation)
  • Rapid procedure:
  • Convenient for a large number of immunoprecipitation: up to 100 per ml

Bio-Adembeads (300nm) coated with recombinant high quality Protein G covalently coupled to the surface. Protein G is cell wall components produced by a Group of G Streptococci. The protein G employed  is recombinant form containing the Fc binding domains without albumin as well as cell wall and cell membrane binding sites to ensure the maximum specific IgG binding. Protyein G binds to all IgG subclasses from human, mouse and rat species. It also binds total IgG from guinea pig, rabbit, goat, cow, sheep and horse.
Immunoprecipitation Cells are lysed and cell debris are removed. A specific antibody is first added to the cell lysate. The Bio-Adembeads Protein G are then incubated with the cell lysate in order to form a magnetic labelled immune complex wich can be easily separated from the liquid by removing the supernatant during the magnetization step. Immunoglobulin purification Bio-Adembeads Protein G are particularly suitable for immunoglobulin purification from ascite, serum, tissue culture supernatants or other samples. After a short incubation time the immunoglobulins are eluted from the Bio-Adembeads Protein G and the containing Ig supernatant is pipetted off for downstream applications.
Diameter 300 nm (CV max 20%)
Magnetic susceptibility approx. 40 emu/g
Specific surface area 10 m2/g
Iron oxide content approx. 70%
Binding capacity ~100µg/ml
Number of reactions 100 immunoprecipitations (Cat No 2004330) / 250 immunoprecipitations (Cat No 2004331)
Storage Supplied in storage buffer (Cat No 1010201) proclin 300 as a preservative Store at 2-8°C. The Bio-Adembeads Protein A must be maintained in liquid during storage and all handling steps. Drying will result in reduced performance. Do not freeze the product.


  • Immunoprecipitation
  • Co-immunoprecipitation
  • Immunoglobulin purification
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