ChIP-Adembeads Protein A + Blocking Buffer for chromatin IP

In stock
SKU
2004240
250,00 €
Magnetic beads for low background Chromatin IP (ChIP). Chemical blocking buffer avoids exogenous DNA. Downstream applications: ChIP on ChIP, sequencing ChIP, RT-PCR, qPCR
£230 / €250

Supplier
Ademtech
Cat. No
2004240
Kit size
40 ChIP
Links
ademtech
Protocol
Information Sheet

Features

  • High efficiency - Effective IP and low non specific binding due to optimized magnetic nanoparticles - ChIP-Adembeads Protein A after incubation with Blocking buffer have a specific surface designed for efficient Protein DNA complex immunoprecipitation and to minimize non-specific enrichment - ChIP-Adembeads combined to Blocking Buffers reduce background - No sedimentation problem
  • Save time and effort - Spin steps have been replaced by rapid magnetic separations, reducing the amount of hands-on time during the assay - Time consuming steps are reduced or eliminated - No pre-clearing step - Capture and direct amplification: go directly to PCR and Real-time PCR without elution step
  • Easy to use ChIP-adem-Kit contains all necessary components for successful monitoring of transcriptions factors and histones / DNA interactions
  • Enhance sensitivity method more effective compare to sepharose beads
  • Ideal for many samples processing

The ChIP-Adem-Kit is an innovative system especially designed for monitoring transcription factors or histones / DNA interactions. The ChIP-Adembeads Protein A after incubation with the Blocking Buffer have a specific surface designed for efficient Protein / DNA complex immunoprecipitation and to minimize non-specific enrichment. Proteins and other contaminants are eliminated in the washing steps. The ChIP protocol is greatly simplified by using blocked Protein A-coated nanoparticles. Some steps have been completely eliminated. Unlike traditional agarose beads, chromatin pre-clearing is not required. ChIP-Adembeads combined to designed buffers reduce background and maximize the amount of templates available for the reaction, achieving greater sensitivity where maximum DNA recovery is critical. The isolated DNA can be used in downstream applications : PCR and Real-Time PCR.
After crosslinking of the cells (or tissue samples) with formaldehyde, the chromatin is sheared by sonication to fragments of 500bp average length. ChIP-Adembeads Protein A are then incubated with our chemical blocking Buffer . The magnetic beads acquire then a specific surface designed for efficient Protein/ DNA complex immunoprecipitation and to minimize non-specific enrichment. The appropriate immunoprecipitating antibody and then the sheared crosslinked chromatin are added to the beads. After incubation, the DNA is eluted from the beads and crosslinking is reversed through proteinase K digestion and incubation at 65°C. After purification, the DNA is ready for downstream applications.
Protein A magnetic beads + Blocking Buffer, 40 ChIP


  • PCR
  • Real-time PCR
  • ChIP-on-chip
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