Circulating & Exosome DNA
Circulating DNA is emerging as a novel non-invasive tool for patient’s stratification and disease monitoring. While most of the research has focused on circulating cell-free (cfDNA) or circulating-tumor-cell-(CTC)-derived DNA, extracellular vesicle-(EVs)-associated DNA (EV-DNA) is emerging as a third valuable “liquid biopsy” platform. In fact, genomic single or double-stranded DNA and mitochondrial DNA have been recently detected in extracellular vesicles. In particular, the majority of double-stranded DNA seems to be associated with tumor derived exosomes (Thakur BK et al. 2014; Kalhert et al. 2014) where it represents the entire genome of the cancerous tumor from which exosomes were derived. This discovery corroborates the potential of exosomes, which can be easily obtained from a simple blood test.
The new EXO-DNAc Kit combines the ability of our reagent DNA-Prep to co-isolate EVs and circulating DNA from biofluids (plasma, urine, serum etc..) or culture supernatants with a user-friendly system for DNA purification. Isolated vesicles are lysed with the appropriate lysis buffer and DNA is purified by spin columns and optimized buffers with a fast turnaround time (approximatively 30 minutes). Finally, EXO-DNAc provides an appropriated DNA concentrator for concentrating and increasing the yield and purity of the DNA to the high levels required for digital PCR analysis and NGS.
Applications: Discovering of mutations by qPCR or digital PCR. Direct exosome capture and DNA purification from biofluids of cell media without time consuming purification steps. Isolation and profiling of genomic EV-associated DNA by DNAse treatment.
Advantages: Highly pure circulating and EVs-associated DNA. Fast and easy protocol. Small volume amount required. Suitable for concomitant profiling of cell-free circulating DNA and EV-associated DNA. The only kit on the market providing Exosome Standards as control.