KineticBlue™ Fluorometric Cell Viability Assay

In stock
SKU
5001001
280,00 €
KineticBlue™ Fluorometric Cell Viability Assay Kit offers a simple, rapid, reliable, sensitive, safe and cost-effective measurement of cell viability. Kinetic Blue™ detects cell viability by utilizing a blue and nonfluorescent dye resazurin, which is conv
£256 / €280


Supplier
Krishgen BioSystems
Cat. no.
5001001
Kit size
100ml
Links
Krishgen BioSystems
Protocol
Resource

Features

  • Allows to assess cells health prior, after or during living cells based assays
  • single step method allowing detection of cell viability by utilizing a blue and nonfluorescent dye resazurin, which is converted to a pink and fluorescent dye resorufin: allows for both fluorometric (more sensitive) or colorimetric detection
  • This assay is as sensitive as thymidine assay for detecting cell proliferation: Depending on the cell types, Kinetic Blue™ can detect as few as 40 cells with reproducible and sensitive signal.

KineticBlueTM Fluorometric Cell Viability Assay Kit offers a simple, rapid, reliable, sensitive, safe and cost-effective measurement of cell viability. KineticBlueTM detects cell viability by utilizing a blue and nonfluorescent dye resazurin, which is converted to a pink and fluorescent dye resorufin in response to chemical reduction of growth medium resulting from cell growth. Continued cell growth maintains a reduced environment while inhibition of growth maintains an oxidized environment. Reduction related to growth causes the REDOX indicator to change from the oxidized (nonfluorescent, blue) form to the reduced (fluorescent, red) form. The fluorescent signal is monitored using 530-560 nm excitation wavelength and 590 nm emission wavelength. The absorbance is monitored at 570 nm and 600 nm. For optimal result, subtract background OD at 600 nm from OD at 570 nm. The fluorescent and colorimetric signal generated from the assay is proportional to the number of living cells in the sample. The KineticBlueTM assay is designed to quantitatively measure the proliferation of various human and animal cell lines, bacteria and fungi.
Protocol:
  • Standard Curve
  • 1. Plate cells in 100µL medium into 96-well tissue culture plates by conducting cell number titration in the range of 40 to 10,000 for adherent cells and 2,000 to 500,000 for suspension cells. For background control, use 100µL medium without cells.
    2. Add 10µL KineticBlueTM solution into medium and incubate cells at 37oC overnight.
    3.Measure absorbance at 570 nm and 600 nm or fluorescence with excitation wavelength at 530 nm and emission wavelength at 590 nm using a micro-titer plate reader.
    4.Obtain OD570-OD600 for each sample if colorimetric detection method is chosen, or fluorescence signal from each sample deducted by background fluorescence from the background control, and plot a standard curve to identify the optimal cell concentration for your assay.
  • KineticBlueTM Fluorometric Cell Viability Assay
    1.Plate cells into 96-well tissue culture plates using optimal cell concentration.
    2.Carry out your experiment by adding agents of your interest into appropriate well and incubate with cells for a certain period of time.
    3.Add 10µL KineticBlueTM solution into medium and incubate cells at 37oC overnight.
    4.Measure absorbance at 570 nm and 600 nm or fluorescence with excitation wavelength at 530 nm and emission wavelength at 590 nm using a micro-titer plate reader.
    5.Obtain OD570-OD600 for each sample if colorimetric detection method is chosen, or fluorescence signal from each sample deducted by background fluorescence from the background control.
  • Kit Component
  • 100mL KineticBlue™ solution (sterile)
  • Storage and Handling
  • Upon receipt, the kit should be stored at 4°C and protected from light. Stored properly, the kit components should remain stable for 6 months.
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