TAL Effector Nucleases

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TAL Effector Nucleases
0,00 €

Availability: In stock

SKU : TALENs

TAL Effector nucleases (TALENs) are artificial restriction enzymes, created by fusing an engineered TAL DNA binding domain
(which targets a desired sequences) with the DNA cleavage domain of FokI, a type II restriction enzyme.
Description

Details

Supplier
LabOmics-ToolGen
Product
TALEN pairs service

Features

  • Genome Engineering: Gene knock-out, insertion, mutation, correction or deletion

  • Targeted Genome edition: Designed to target precise, specific locus

  • Complete knockout of any target gene

  • No antibiotic selection required

  • Biallelic modifications occur frequently

  • Ready to use: No labor-intensive E. coli-based pre-screening steps

  • Validated activity in mammalian cells

  • Affordable price

.TALENs are engineered DNA-cleaving enzymes made by fusing tailor-made DNA-binding peptidic modules to the FokI nuclease domain. TALENs induce site-specific double strand breaks (DSBs) in DNA at user-defined locations, which leads to targeted modification of the genome. Each TALE module recognizes a single nucleotide base. The fusion of 15-20 modules gives an unsurpassed sequence specificity to each TALE, and the use of a TALE pair is then considerably accurate. Recent publications have established TALENs as an innovative tool for targeted genome editing in diverse cell lines and organisms (method of the year 2011 - Nature Methods Jan 2012). Because the DNA-binding domain can be designed to recognize and introduce DNA double-strand breaks specifically in desired sequences in the genome, TALENs provide an innovative tool for in vivo genome editing. Using our custom-synthesized TALENs, scientists can knock out any gene of interest or edit its DNA sequence in cultured cells or other model systems such as zebrafish, mouse, rat, plants, etc.





Fig. 1. Principle of TALEN targeting and cleavage.
TALENs make various types of genome editing possible, including, but not limited to, (1) the introduction of mutations into a gene of interest to knockout its function, (2) the deletion or insertion of genetic information at specific loci, and (3) the induction of large scale genetic deletions, duplications, and inversions. These types of genome editing were difficult, if not impossible, to achieve before ZFNs and TALENs were developed; the ability to undertake such editing represents a unique opportunity in both basic and pharmaceutical research to translate our growing understanding of the genetic basis of many biological and pathological processes into industrial and therapeutic breakthroughs.

The TALEN service includes the design of TALENs that target your gene of interest using our proprietary computer algorithm and synthesis of a carefully chosen set of TALENs. Our TALEN custom service will allow you to start your experiments with a set of well-designed, ready-to-use TALENs, bypassing the need for labor-intensive TALEN design and synthesis efforts in your own lab.


The TALEN service includes the design of TALENs that target your gene of interest using our proprietary computer algorithm and synthesis of a carefully chosen set of TALENs. Our TALEN custom service will allow you to start your experiments with a set of well-designed, ready-to-use TALENs, bypassing the need for labor-intensive TALEN design and synthesis efforts in your own lab.
Genome Editing

-Gene Knock-out

-Gene Knock-out / Tagging

-Gene Insertion

-Gene Deletion

-Gene Mutation

-Gene Correction

1. Identify the gene target of interest:
Gather as much as possible of the following information: Gene Description, species, Nucleotide Accession, Entrez Gene ID, Symbols/Aliases or Catalog/product ID. You can also submit a sequence so we can search by target gene sequences homology (BLAST)



2. Fill out the order form:

and fill with requested information. Send it back to us by email or FAX. We will contact you shortly to confirm: sequences, vectors and related products

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