Plant/Fungi DNA Isolation Kit

In stock
SKU
1026200
201,00 €
Plant/Fungi DNA Isolation Kit
Supplier
Norgen Biotek
Cat. no.
1026200
Kit size
50
Links
Norgen Biotek
Protocol
Information Sheet

Features

  • No liquid nitrogen or physical disruption methods required - Liquid nitrogen is not required for homogenization of samples, making DNA purification rapid and convenient.
  • Rapid and simple processing - Rapid spin-column format allows for the processing of multiple samples in 45 minutes, while the 96-well plates can be processed in 30 minutes.
  • High yield of DNA - Plus, the purified DNA is of the highest quality and can be used in a number of sensitive downstream applications, including PCR, qPCR, Southern blotting, SNP analysis and Sequencing
  • Isolate DNA from a wide range of samples - DNA can be isolated from a wide range of plant and filamentous fungi samples.
  • Isolate total DNA, including viral DNA - Purified DNA samples can be used for the detection of viral pathogens, as viral DNA is isolated with the plant/fungi DNA.
  • No phenol:chloroform extractions
  • 96-well format available - This kit is also available in a 96-well format for high throughput applications

Norgen’s Plant/Fungi DNA Isolation Kit provides a rapid method for the isolation and purification of total DNA from a wide range of plant and filamentous fungal species. Total DNA, including genomic DNA, mitochondrial DNA and chloroplast DNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit.

The procedure is rapid and convenient, as it does not rely on the use of liquid nitrogen in order to homogenize the samples.

The purified DNA is of the highest integrity, and can be used in a number of downstream applications including real - time PCR, SNP, Southern blotting and sequencing.

Norgen's Plant/Fungi DNA Isolation Kit is also available in a 96-well format for high throughput applications. Purification with the 96-well plates can be performed using either a vacuum manifold or centrifugation.
Briefly, the cells are macerated using the provided Lysis Solution, and the released DNA is bound to Norgen's column in the presence of Binding Solution. Under these conditions only the DNA will bind to the resin while most of the contaminating RNA and proteins are removed in the flowthrough. The bound DNA is then washed to remove any remaining impurities. Lastly, the purified DNA is eluted into 50-100 µL of the provided Elution Buffer or water.

The purified DNA is of the highest integrity, and can be used in a number of downstream applications including real - time PCR, SNP, Southern blotting and sequencing.
Column Binding Capacity 50 µg
Maximum Column Loading Volume 650 µL
Maximum Amount of Starting Material Plant Tissues: 50 mg Plant Cells: 50 mg Fungi (wet weight): 50 mg
Average Yields 50 mg Tomato Leaves: 18 µg 50 mg Grape Leaves: 10 µg 50 mg Peach Leaves: 10 µg 50 mg Plum Leaves: 10 µg
50 mg Pine Needles: 5 µg Botrytis cinerea (50 mg wet weight): 1.5 µg Fusarium sp. (50 mg wet weight): 2 µg Aspergillus niger (50 mg wet weight): 4 µg
Time to Complete 10 Purifications 45 minutes
Isolate DNA from a Wide Range of Plants.
DNA was isolated from 50 mg samples of tobacco leaves (Lanes 1 and 2), tomato leaves (lanes 3 and 4), peach leaves (Lanes 5 and 6) and grape leaves (lanes 7 and 8) using Norgene’s Plant/Fungi DNA Isolation Kit, and 5 µL aliquots of the 100 µL elutions were run on a 1x TAE 1% agarose gel. As it can be seen, high quality DNA was isolated in all cases. The M lanes contain Norgen’s HighRanger 1Kb DNA Ladder.


Purified DNA can be Amplified in a Real-time PCR Reaction (SYBR Green)
DNA was isolated from 50 mg samples of plum and tobacco frozen leaves using Norgen’s Plant/Fungi DNA isolation Kit, and 2 µL of the eluted DNA was used in a real-time PCR reaction (total reaction volume 20 µL) with 18sr DNA primers (95ºC for 3min and 40 cycles at 95 ºC for 15 sec. and 60 ºC for 30 sec.). The real-time PCR was successful in amplifying both the plum and tobacco DNA, indicating that the DNA is of a high quality and can be used in sensitive down steam applications.


  • PCR
  • qPCR
  • Southern Blotting
  • Sequencing
  • SNP analysis
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