EXO-Prep is a fast and convenient method of exosome isolation from biofluids and cell culture supernatants. Isolation with EXO-Prep is based on chemical precipitation. Samples are incubated with EXO-Prep solution on ice, so that exosomes will precipitate following centrifugation. The obtained pellet can be resuspended in PBS 1X or deionized water. The protocol is user friendly, timesaving (around 1 hour), and does not require capital laboratory equipment. Isolated exosomes are suitable for a wide range of analyses, such as NTA, protein profiling by using different techniques (western blotting, ELISA, FACS), nucleic acid extraction and profiling of mRNA or small RNA markers.
Applications: Single step isolation of exosomes from biofluids and cell supernatants. EXO-Prep is able to isolate the overall exosome population in a sample. Isolated exosomes can be used for NTA analysis, protein profiling (WB, FACS, ELISA) and nucleic acid (RNA and DNA) extraction and profiling.
Advantages: No ultracentrifugation required. Easy and fast protocol. EXO-Prep is able to isolate exosome from small volumes of sample (as low as 100 μl of plasma).Easy to store and ship (4°C).